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XBridge BEH Phenyl Column

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苯基鍵合相；特別適用于極性芳香化合物

詳細(xì)介紹

XBridge BEH Phenyl 色譜柱
? 高化學(xué)穩(wěn)定的Ⅱ 型苯基柱
? 對芳香化合物有的選擇性
? 三鍵鍵合方式，有效提高苯基抗流失性，避免苯基流失本底峰（UV），兼容LC/MS
? 溫度上限：低pH時80℃，高pH時60℃


BEH顆粒能夠顯著改善色譜峰形的關(guān)鍵因素是其在合成工藝中 采用了亞乙基橋結(jié)構(gòu)。亞乙基橋連接相鄰的兩個硅醇基，這 不僅增加了顆粒強度，同時也減少了游離的硅醇基位點，從 而減少硅醇基與樣品間不利的相互作用力。傳統(tǒng)的方法(例如 大量的封端(excessive end capping))于封端試劑的空間位阻 效應(yīng)及在活性位點上連接配體。這樣，殘余的游離硅醇基暴 露后就會導(dǎo)致峰展寬和峰拖尾。亞乙基橋可減少游離的硅醇 基數(shù)量，使分子空間排布配體鍵合及封端?？刂七@一 過程可有助于XBridge色譜柱得到的峰形。

色譜柱在低pH流動相中柱壽命差的主要原因是鍵合相在酸性條件下發(fā)生水解。XBridge填料采用沃特世專有的、的鍵合技術(shù)與封端技術(shù)，可以在低pH條件下表現(xiàn)出良好的配體穩(wěn)定性與色譜重現(xiàn)性。

Advantages of CORTECS C18 2.7 μm and XBridge BEH Phenyl X P 2.5 μm Columns for the Analysis of a Comprehensive Panel of Pain Management Drugs for Forensic Toxicology

In forensic toxicology, drug screening panels often include such commonly used substances such as opiates, benzodiazepines and stimulants. These panels are often analyzed by LC-MS using traditional C18 column technologies. Key considerations include the ability to chromatographically resolve the various pairs of isobaric compounds included in these panels, while maintaining good peak shape for a variety of compounds. In addition, when using traditional HPLC systems, the ability to analyze samples as rapidly as possible without exceeding the pressure limitations of the system is very important. This application note highlights the capabilities of Waters’ new CORTECS C18 2.7 μm Columns and XBridge BEH Phenyl XP 2.5 μm Columns for this type of application. In the case of the CORTECS C18 Column, the high efficiency packing of solid core 2.7 μm particles yields excellent performance that equals or exceeds competitive columns at lower operating backpressures. If alternative selectivity is desired, the phenyl functionality of the BEH phenyl column enhances the retention of opiate compounds. This enhanced retention can potentially result in reducing ion suppression from urinary matrix components. Both columns achieve excellent baseline separation between isomers, and the entire panel of 35 compounds, including opioids, benzodiazepines, stimulants, and other drugs of abuse can be analyzed in under 4 minutes at backpressures compatible with any HPLC system

品名

編號

XBridge BEH Phenyl Column, 130?, 5 µm, 4.6 mm X 100 mm, 1/pkg

186003351

XBridge BEH Phenyl Column, 130?, 3.5 µm, 4.6 mm X 30 mm, 1/pkg

186003331

XBridge BEH Phenyl Column, 130?, 3.5 µm, 4.6 mm X 50 mm, 1/pkg

186003332

XBridge BEH Phenyl Column, 130?, 3.5 µm, 4.6 mm X 75 mm, 1/pkg

186003333

XBridge BEH Phenyl Column, 130?, 5 µm, 2.1 mm X 100 mm, 1/pkg

186003339

XBridge BEH Phenyl Column, 130?, 5 µm, 2.1 mm X 50 mm, 1/pkg

186003338

XBridge BEH Phenyl Column, 130?, 3.5 µm, 1 mm X 50 mm, 1/pkg

186003317

XBridge BEH Phenyl Column, 130?, 3.5 µm, 4.6 mm X 150 mm, 1/pkg

186003335

XBridge BEH Phenyl Column, 130?, 3.5 µm, 4.6 mm X 100 mm, 1/pkg

186003334

XBridge BEH Phenyl Column, 130?, 5 µm, 2.1 mm X 30 mm, 1/pkg

186003337

XBridge BEH Phenyl Column, 130?, 3.5 µm, 4.6 mm X 250 mm, 1/pkg

186003965

XBridge BEH Phenyl Column, 130?, 5 µm, 2.1 mm X 150 mm, 1/pkg

186003340

XBridge BEH Phenyl Column, 130?, 5 µm, 3 mm X 100 mm, 1/pkg

186003344

XBridge BEH Phenyl Column, 130?, 5 µm, 3 mm X 50 mm, 1/pkg

186003343

XBridge BEH Phenyl Column, 130?, 5 µm, 3 mm X 30 mm, 1/pkg

186003342

XBridge BEH Phenyl Column, 130?, 3.5 µm, 2.1 mm X 30 mm, 1/pkg

186003321

XBridge BEH Phenyl Column, 130?, 3.5 µm, 2.1 mm X 50 mm, 1/pkg

186003322

XBridge BEH Phenyl Column, 130?, 3.5 µm, 3 mm X 150 mm, 1/pkg

186003329

XBridge BEH Phenyl Column, 130?, 3.5 µm, 3 mm X 100 mm, 1/pkg

186003328

XBridge BEH Phenyl Column, 130?, 3.5 µm, 3 mm X 50 mm, 1/pkg

186003327

XBridge BEH Phenyl Column, 130?, 3.5 µm, 3 mm X 30 mm, 1/pkg

186003326

XBridge BEH Phenyl Column, 130?, 3.5 µm, 2.1 mm X 150 mm, 1/pkg

186003324

XBridge BEH Phenyl Column, 130?, 3.5 µm, 2.1 mm X 100 mm, 1/pkg

186003323

XBridge BEH Phenyl Column, 130?, 5 µm, 3 mm X 150 mm, 1/pkg

186003345

XBridge BEH Phenyl Column, 130?, 5 µm, 3 mm X 250 mm, 1/pkg

186003346

XBridge BEH Phenyl Column, 130?, 5 µm, 4.6 mm X 75 mm, 1/pkg

186003350

XBridge BEH Phenyl Column, 130?, 5 µm, 4.6 mm X 250 mm, 1/pkg

186003353

XBridge BEH Phenyl Column, 130?, 5 µm, 4.6 mm X 150 mm, 1/pkg

186003352

XBridge BEH Phenyl Intelligent Speed (IS) Column, 130?, 3.5 µm, 4.6 mm X 20 mm, 1/pkg

186003330

XBridge BEH Phenyl Method Validation Kit, 130?, 2.5 µm, 4.6 mm X 150 mm, 3/pkg

186006778

XBridge BEH Phenyl Method Validation Kit, 130?, 2.5 µm, 3 mm X 150 mm, 3/pkg

186006777

XBridge BEH Phenyl Method Validation Kit, 130?, 2.5 µm, 2.1 mm X 150 mm, 3/pkg

186006776

XBridge BEH Phenyl Intelligent Speed (IS) Column, 130?, 5 µm, 2.1 mm X 20 mm, 1/pkg

186003336

XBridge BEH Phenyl Intelligent Speed (IS) Column, 130?, 3.5 µm, 2.1 mm X 20 mm, 1/pkg

186003320

XBridge BEH Phenyl Method Validation Kit, 130?, 2.5 µm, 4.6 mm X 50 mm, 3/pkg

186006219

XBridge BEH Phenyl Method Validation Kit, 130?, 2.5 µm, 3 mm X 100 mm, 3/pkg

186006218

XBridge BEH Phenyl Method Validation Kit, 130?, 2.5 µm, 2.1 mm X 100 mm, 3/pkg

186006216

XBridge BEH Phenyl Method Validation Kit, 130?, 2.5 µm, 2.1 mm X 50 mm, 3/pkg

186006215

XBridge BEH Phenyl Method Validation Kit, 130?, 3.5 µm, 2.1 mm X 100 mm, 3/pkg

186003799

XBridge BEH Phenyl Method Validation Kit, 130?, 3.5 µm, 4.6 mm X 150 mm, 3/pkg

186003803

XBridge BEH Phenyl Intelligent Speed (IS) Column, 130?, 3.5 µm, 3 mm X 20 mm, 1/pkg

186003325

XBridge BEH Phenyl Method Validation Kit, 130?, 3.5 µm, 4.6 mm X 100 mm, 3/pkg

186003802

XBridge BEH Phenyl Method Validation Kit, 130?, 3.5 µm, 3 mm X 150 mm, 3/pkg

186003801

XBridge BEH Phenyl Method Validation Kit, 130?, 3.5 µm, 3 mm X 100 mm, 3/pkg

186003800

XBridge BEH Phenyl Intelligent Speed (IS) Column, 130?, 5 µm, 4.6 mm X 20 mm, 1/pkg

186003347

XBridge BEH Phenyl Method Validation Kit, 130?, 5 µm, 4.6 mm X 100 mm, 3/pkg

186003807

XBridge BEH Phenyl Column, 130?, 5 µm, 4.6 mm X 30 mm, 1/pkg

186003348

XBridge BEH Phenyl Method Validation Kit, 130?, 5 µm, 3 mm X 100 mm, 3/pkg

186003805

XBridge BEH Phenyl Method Validation Kit, 130?, 5 µm, 2.1 mm X 150 mm, 3/pkg

186003804

XBridge BEH Phenyl Column, 130?, 5 µm, 4.6 mm X 50 mm, 1/pkg

186003349

XBridge BEH Phenyl OBD Prep Column, 130?, 5 µm, 30 mm X 50 mm, 1/pkg

186003277

XBridge BEH Phenyl OBD Prep Column, 130?, 5 µm, 30 mm X 75 mm, 1/pkg

186003278

XBridge BEH Phenyl Prep Guard Cartridge, 130?, 5 µm, 19 mm X 10 mm, 1/pkg

186003355

XBridge BEH Phenyl OBD Prep Column, 130?, 5 µm, 30 mm X 150 mm, 1/pkg

186003276

XBridge BEH Phenyl Prep Guard Cartridge, 130?, 5 µm, 10 mm X 10 mm, 1/pkg

186003354

XBridge BEH Phenyl XP Column, 130?, 2.5 µm, 2.1 mm X 150 mm, 3/pkg

176002885

XBridge BEH Phenyl Prep Guard Cartridge, 130?, 5 µm, 30 mm X 10 mm, 1/pkg

186006891

XBridge BEH Phenyl OBD Prep Column, 130?, 5 µm, 50 mm X 100 mm, 1/pkg

186003940

XBridge BEH Phenyl XP Column, 130?, 2.5 µm, 2.1 mm X 30 mm, 1/pkg

186006064

XBridge BEH Phenyl OBD Prep Column, 130?, 5 µm, 19 mm X 50 mm, 1/pkg

186003356

XBridge BEH Phenyl OBD Prep Column, 130?, 5 µm, 19 mm X 100 mm, 1/pkg

186003357

XBridge BEH Phenyl OBD Prep Column, 130?, 5 µm, 19 mm X 150 mm, 1/pkg

186003358

XBridge BEH Phenyl OBD Prep Column, 130?, 5 µm, 30 mm X 100 mm, 1/pkg

186003279

XBridge BEH Phenyl XP Column, 130?, 2.5 µm, 2.1 mm X 100 mm, 1/pkg

186006067

XBridge BEH Phenyl XP Column, 130?, 2.5 µm, 2.1 mm X 150 mm, 1/pkg

186006718

XBridge BEH Phenyl OBD Prep Column, 130?, 5 µm, 50 mm X 50 mm, 1/pkg

186003936

XBridge BEH Phenyl OBD Prep Column, 130?, 5 µm, 19 mm X 250 mm, 1/pkg

186004024

XBridge BEH Phenyl Method Validation Kit, XP Column, 130?, 2.5 µm, 3 mm X 50 mm, 3/pkg

186006217

XBridge BEH Phenyl VanGuard Cartridge, 130?, 5 µm, 3.9 mm X 5 mm, 3/pkg

186007798

XBridge BEH Phenyl XP Column, 130?, 2.5 µm, 2.1 mm X 100 mm, 3/pkg

176002573

XBridge BEH Phenyl VanGuard Cartridge, 130?, 5 µm, 2.1 mm X 5 mm, 3/pkg

186007796

XBridge BEH Phenyl VanGuard Cartridge, 130?, 3.5 µm, 3.9 mm X 5 mm, 3/pkg

186007795

XBridge BEH Phenyl Method Validation Kit, 130?, 5 µm, 4.6 mm X 250 mm, 3/pkg

186003809

XBridge BEH Phenyl VanGuard Cartridge, 130?, 3.5 µm, 2.1 mm X 5 mm, 3/pkg

186007793

XBridge BEH Phenyl Method Validation Kit, 130?, 5 µm, 4.6 mm X 150 mm, 3/pkg

186003808


關(guān)鍵詞：色譜柱
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