BDP FL tetrazine,氟化硼二吡咯-四嗪

Inverse electron demand Diels-Alder reaction (ied-DA) with tetrazines is a promising tool for the conjugation of biomolecules. The reaction takes place between tetrazine as an electron acceptor heterodiene, and a strained dienophile, such as trans-cyclooctene, cyclopropene, or some cyclooctynes.

Methyltetrazine provides greater stability in buffers than unsubstituted tetrazine. The rate of its reaction with cycloalkenes still beats almost all other conjugation reaction rates by a factor of magnitudes.

BDP FL is a bright dye for fluorescein (FAM) channel. Using BDP FL tetrazine, the dye can be conjugated with molecules bearing strained olefins.

General properties

Spectral properties

BDP FL tetrazine,氟化硼二吡咯-四嗪

馬來酰亞胺 Dye maleimides

Pyrene maleimide

BDP FL maleimide

Cyanine3 maleimide

Cyanine5 maleimide

Cyanine5.5 maleimide

Cyanine7 maleimide

Cyanine7.5 maleimide

Sulfo-Cyanine3 maleimide

Sulfo-Cyanine5 maleimide

伯胺 Primary amine

BDP FL amine

Cyanine3 amine

Cyanine5 amine

Cyanine5.5 amine

Cyanine7 amine

Cyanine7.5 amine

Sulfo-Cyanine5 amine

Sulfo-Cyanine7 amine

酰肼 Dye hydrazides

Pyrene hydrazide

BDP FL hydrazide

Cyanine3 hydrazide

Cyanine5 Boc-hydrazide

Cyanine5 hydrazide

Cyanine5.5 hydrazide

Cyanine7 hydrazide

Cyanine7.5 hydrazide