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上海懋康生物科技有限公司
中級會員 | 第10年

18616957973

生化試劑
抗腫瘤化合物/免疫抑制劑 常用生化試劑 氨基酸及蛋白質 表面活性劑 生物試劑 植物生長調節(jié)劑 小分子抑制劑 病理染色液 蛋白/磷酸酶抑制劑 去垢劑 維生素/輔酶 脂糖類 腐草霉素 Phleomycin (LPS) 脂多糖 DO Supplement 缺陷培養(yǎng)基 潮霉素B Hygromycin B 嘌呤霉素 Puromycin 硫酸諾爾絲菌素Nourseothricin Aureobasidin A (AbA)金擔子素A 核酸及衍生物 染色劑/指示劑 前列腺素類 生物分子 微生物培養(yǎng) Timentin * Glufosinate Ammonium 草銨膦 Aureobasidin A 金擔子素A 核酸凝膠染料 DTT 二硫蘇糖醇 Rapamycin 雷帕霉素 Tunicamycin 衣霉素 Thiostrepton硫鏈絲菌素 Blasticidin S 殺稻瘟菌素S Bialaphos 雙丙氨膦鈉鹽 rac-GR24 獨腳金內酯 乙酰神經(jīng)氨酸 緩沖劑與緩沖溶液 Streptozotocin (STZ) 鏈脲佐菌素 其他生化試劑 抗生素/抗真菌素
分子生物學
免疫學
細胞生物學
蛋白質研究
耗材
信號轉導研究相關
酵母單/雙雜交研究

霍亂毒素(Cholera Toxins,CTX)產(chǎn)品專題

時間:2018/2/5閱讀:10304
分享:

霍亂毒素(Cholera Toxins,CTX)

 

 

同義名:

Cholera enterotoxin; Choleragen; 霍亂腸毒素;霍亂腸菌素;

 

搜索關聯(lián)詞:

霍亂毒素(Cholera toxin); 霍亂類毒素Choleragenoid;霍亂毒素B亞基(CTB);霍亂毒素B亞單位;腺苷酸環(huán)化酶(Adenylate cyclase,AC);Forskolin 佛司可林;

 

基本介紹:

霍亂毒素(Cholera toxin,CTX)是霍亂弧菌產(chǎn)生的毒力因子,會引起嚴重腹瀉以及人體脫水。CTX是一種分子量約84kDa的寡蛋白,包含一個A亞基以及包圍在周圍的5個B亞基構成。CTX是一種有效的腺苷酸環(huán)化酶(Adenylate cyclase,AC)活化劑,也是負責霍亂癥狀的病原物質?;魜y毒素B亞基(a.k.a. 霍亂類毒素choleragenoid)負責全毒素識別并結合到哺乳動物細胞表面的GM1神經(jīng)節(jié)苷脂受體,促進A亞基進入細胞;A亞基承擔ADP核糖轉移酶(ADP-ribosyl-transferase)活性,此酶能下調Gs蛋白表達并活化AC酶,進而促進cAMP水平提高。A亞基還能ADP核糖基化視桿細胞外節(jié)膜盤的轉運蛋白,使得GTPase失活。歸因于GM1神經(jīng)節(jié)苷脂受體普遍存在于真核細胞膜上,CTX用于各種各樣的模型系統(tǒng)中活化腺苷酸環(huán)化酶(AC)。CTX還是一種的黏膜疫苗佐劑,通過抑制IL-12生產(chǎn)來誘導2型輔助性T細胞的免疫應答。

 

生理功能:

霍亂毒素(Cholera toxin,CTX)由霍亂弧菌(Vibrio cholerae)代謝純化所得,不僅在微生物學是一種強大的研究工具,還普遍用于生理學、細胞生物學和生物化學。常見應用如下:

1)因其對腺苷酸環(huán)化酶(AC)的效應,CTX通常用來研究信號轉導機制。

2)CTX通過刺激B淋巴細胞活性用作一種佐劑;(與疫苗佐劑的開發(fā)有關,CTX有效調節(jié)哺乳動物細胞免疫系統(tǒng),用作一種聯(lián)合抗原共同注射的佐劑)

3)純化的霍亂毒素B亞基(Cholera toxin B subunit)用于神經(jīng)研究方面的神經(jīng)示蹤,利用其GM1 神經(jīng)節(jié)苷脂結合和逆行轉運特點。

 

基本參數(shù):

1)CAS NO:9012-63-9

2)純度:>95%(When examined by polyacrylamide gel electrophoresis in a non-denaturing system run at alkaline pH, this protein migrates as a single major band. When examined on SDS-PAGE, all subunits, A, A1, A2, and B, are present. Densitometric analysis estimates the purity as >95%. The 280 to 260 nm ratio of absorbance (R280/260) is 2.

3)內毒素:~5 EU/mg (determined using a kinetic chromogenic LAL assay)

4)生物活性:Hemagglutination activity is assessed with trisialoganglioside fixed sheep red blood cells using a modification of the method described by Sato, et al. This lot met the specification of exhibiting hemagglutination activity at a toxin dilution ≤1.6µg/ml.

 

產(chǎn)品信息

List labs提供凍干粉形式的霍亂毒素(Cholera Toxin,CTX,CT)和霍亂毒素B亞基,所有產(chǎn)品經(jīng)過內毒素去除和無菌處理。并經(jīng)過嚴格的純度和生物活性檢測,是高質量的研究工具。部分現(xiàn)貨供應,*,咨詢maokangbio。

品牌

產(chǎn)品名稱

貨號       

規(guī)格       

List Biological

laboratories      

Cholera Toxin from Vibrio cholerae

101B

1mg

Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

100B

1mg

Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae

103B

1mg

Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt  

104

0.5mg

 

延伸產(chǎn)品:部分現(xiàn)貨供應,*,咨詢maokangbio。

品牌

產(chǎn)品名稱

貨號         

規(guī)格         

List Biological

laboratories      

Cholera Toxin B Subunit (CTB) from Vibrio cholerae FITC, Conjugate     

106

0.2mg

Cholera Toxin B Subunit Biotin Conjugate

112

0.2mg

Anti-Cholera Toxin B Subunit (Goat)

703

0.1ml

【應用說明】:

1)霍亂毒素B亞基(CTB)偶聯(lián)物用于軸漿運輸和神經(jīng)元連接研究中的組織化學標志物(histochemical markers)。

2)Goat Anti-Cholera Toxin B Subunit (Choleragenoid)適用于中和(Neutralization)和結合實驗,或與未標記霍亂毒素B亞基聯(lián)合的免疫組化研究。

 

引用文獻【List Biological】

Wu J et al. Hydrogen postconditioning promotes survival of rat retinal ganglion cells against ischemia/reperfusion injury through the PI3K/Akt pathway. Biochem Biophys Res Commun. 2018 Jan 22;495(4):2462-2468. PubMed ID: 29288664 [Retinal ganglion cells (RGCs)density was counted using haematoxylin and eosin (HE) staining, retrograde labelling with cholera toxin beta (CTB) and TUNEL staining, respectively. List Biological Cholera Toxin B Subunit (CTB) from Vibrio cholerae FITC, Conjugate (#106)]

 

Aoki S et al. Multizonal Cerebellar Influence Over Sensorimotor Areas of the Rat Cerebral Cortex. Cereb Cortex. 2017 Dec 29. PMID: 29300895 [Under visual guidance, viral solution containing the French subtype of CVS-11 strain of RABV (titrating 4 × 107 plaque-forming units/ml) in conjunction with cholera toxin β-subunit (CTb, low salt; List Biological Laboratories, 1% w/v in 0.2 M phosphate buffer. List Biological Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt (#104) ]

 

Dong L et al. Calcium channels are involved in EphB/ephrinB reverse signaling?induced apoptosis in a rat chronic ocular hypertension model. Mol Med Rep. 2018 Feb;17(2):2465-2471. PMID: 29207174 [Retinal sections were then blocked for 2 h in 6% normal donkey serum, 1% bovine serum, and 0.2% Triton X-100, and dissolved in PBS at room temperature. Consequently, sections were incubated with the following primary antibodies at 4°C for 24 h: polyclonal rat anti-Cav3.1, anti-Cav3.2, (1:200 dilution; Alomone Labs, Jerusalem, Israel), Goat anti-CTB (cat. no. 703; List Biological Laboratories)]

 

Konno K et al. Cell-type specific parallel circuits in the bed nucleus of the stria terminalis and the cenral nucleus of the amygdala of the mouse. J Neurosci. 2014 May 28;34(22):7412-24. PMID: 24872547 [CTb Goat, antiserum 1:3000 choleragenoid Cat. #703, List Biological Laboritories]

 

Lu X et al. Effects of 1,25 and 24,25 Vitamin D on Corneal Epithelial Proliferation, Migration and Vitamin D Metabolizing and Catabolizing Enzymes. Sci Rep. 2017 Dec 5;7(1):16951.PMID: 29208972 [Each cornea was cut in half and placed in a 35 mm dish with the epithelial side up. After approximay 5 minutes in the laminar air-flow culture hood, the half corneal button attached itself to the bottom of the plate, and 1.5 mL of DMEM with 10% serum containing 40 μg/mL gentamicin, 1% ITS, and100 ng/mLcholera toxin(LIST Biological Laboratories, Inc., Campbell, CA) were added and the tissue was placed in a humidified incubator at 37 °C with 5% CO2. Culture medium was replaced every 2 days.]

 

Bruxelle JF et al. Clostridium difficile flagellin FliC: Evaluation as adjuvant and use in a mucosal vaccine against Clostridium difficile. PLoS One. 2017 Nov 27;12(11):e0187212. PMID: 29176760 [Vaccination regimen with ovalbumin as antigen: Ovalbumine (OVA) is a commonly used antigen in order to assess adjuvant activity. In two distinct assays, a total of fourteen mice per group received inra-rectally (IR) 20μg of ovalbumin (ovalbumin grade VII, Sigma) with 7μg of adjuvant, either cholera toxin (CT) (List Biological Laboratories, INC)]

 

注意事項

1)本品僅用作科研研究,由專業(yè)的科學人士,并在正規(guī)的實驗室內操作。絕不可用作其他用途(比如臨床藥物、獸藥、診斷用途等)??!

2)為了您的安全和健康,請穿實驗服并戴一次性手套操作。

  

 — —Written/Edited by V. Shallan【版權歸MKBio懋康所有】

 

上海懋康生物科技有限公司是一家涉足于生命科學和生物技術領域研究的試劑、儀器和實驗室消耗品與實驗服務工作,主要從事細胞生物學、植物學、分子生物學、免疫學、生物化學、蛋白組學。生物制藥與診斷試劑研發(fā)生產(chǎn)等領域。 本公司秉承“以人為本,以誠為信、合同守信”的經(jīng)營理念。堅持"品質保障"的原則為廣大客戶提供產(chǎn)品。

 

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