大鼠6酮前列腺素F1a(6-keto-PGF1a)ELISA試劑盒
實驗目的：本試劑盒僅供研究使用，用于測定大鼠血清，血漿及相關(guān)液體樣本中6酮前列腺素F1a(6-keto-PGF1a)的含量。
實驗原理：本試劑盒應用雙抗體夾心法測定標本中大鼠6酮前列腺素F1a(6-keto-PGF1a)水平。用純化的大鼠6酮前列腺素F1a(6-keto-PGF1a)抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入6酮前列腺素F1a(6-keto-PGF1a)，再與HRP標記的6酮前列腺素F1a(6-keto-PGF1a)抗體結(jié)合，形成抗體-抗原-酶標抗體復合物，經(jīng)過*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的6酮前列腺素F1a(6-keto-PGF1a)呈正相關(guān)。用酶標儀在450nm波長下測定吸光度（OD值），通過標準曲線計算樣品中大鼠6酮前列腺素F1a(6-keto-PGF1a)濃度。
Generic Name：Rat 6-keto-PGF1a ELISA Kit.
Purpose
This kit allows for the determination of 6-keto-PGF1a concentrations in Rat serum, blood plasma, and other biological fluids.
Principle of the assay
The kit assay Rat 6-keto-PGF1a level in the sample，use Purified Rat 6-keto-PGF1a antibody to coat microtiter plate wells, make solid-phase antibody, then add 6-keto-PGF1a to wells, Combined 6-keto-PGF1a antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 6-keto-PGF1a in the samples is then determined by comparing the O.D. of the samples to the standard curve.
上海華壹生物科技有限公司
Shanghai HuaYi Bio-technology Co.,Ltd
