免疫球蛋白M（IgM）檢測試劑盒（血清.血漿.組織）Human Immunoglobulin M,IgM ELISA Kit 齊一生物科技（上海）有限公司以真誠、主動、周到、規(guī)范、熱情的服務(wù)很快得到廣大客戶的*好評.公司秉承“適應(yīng)多樣化的需求,提供專業(yè)化的服務(wù)"的經(jīng)營理念,為客戶提供*的服務(wù)

免疫球蛋白M（IgM）檢測試劑盒（血清.血漿.組織）類別：酶聯(lián)免疫分析檢測試劑盒
規(guī)格：96T/48T
包裝：盒
檢測原理：采用雙抗夾心法
保質(zhì)期：六個月
產(chǎn)品保存條件：2-8°C
應(yīng)用范圍：僅供科研研究實(shí)驗(yàn)使用
樣品形式：血清/血漿/組織勻漿/細(xì)胞培養(yǎng)上清//其它生物液體（具體情況請咨詢）
標(biāo)本的采集及保存：
1.血清：室溫血液自然凝固10-20分鐘后，離心20分鐘左右（2000-3000轉(zhuǎn)/分）。仔細(xì)收集上清。保存  過程 中  如有沉淀形成，應(yīng)再次離心。
2.血漿：應(yīng)根據(jù)標(biāo)本的要求選擇EDTA、檸檬酸鈉或肝素作為抗凝劑，混合10-20分鐘后，離心20分鐘左右（2000-3000轉(zhuǎn)/分）。仔細(xì)收集上清。保存過程中如有沉淀形成，應(yīng)再次離心。
3.尿液：用無菌管收集。離心20分鐘左右（2000-3000轉(zhuǎn)/分）。仔細(xì)收集上清。保存過程中如有沉淀形成，應(yīng)再次離心。胸腹水、腦脊液參照此實(shí)行。
4.細(xì)胞培養(yǎng)上清：檢測分泌性的成份時(shí)，用無菌管收集。離心20分鐘左右（2000-3000轉(zhuǎn)/ 分）。仔細(xì)收集上清。  檢測細(xì)胞內(nèi)的成份時(shí)，用PBS（PH7.2-7.4）稀釋細(xì)胞懸液，細(xì)胞濃度達(dá)到100萬/ml左右。通過反復(fù)凍融，以 使細(xì)胞破壞并 放出細(xì)胞內(nèi)成份。離心20分鐘左右（2000-3000轉(zhuǎn)/分）。仔細(xì)收集上清。保存過程中如有沉淀形成，應(yīng)再次離心。
5.組織標(biāo)本：切割標(biāo)本后，稱取重量。加入一定量的PBS，PH7.4。用液氮迅速冷凍保存?zhèn)溆?。?biāo)本融化后仍然保持2-8℃的溫度。加入一定量的PBS（PH7.4），用手工或勻漿器將標(biāo)本勻漿充分。離心20分鐘左右（2000-3000轉(zhuǎn)/分）。仔細(xì)收集上清。分裝后一份待檢測，其余冷凍備用。
標(biāo)本要求： 
1．標(biāo)本采集后盡早進(jìn)行提取，提取按相關(guān)文獻(xiàn)進(jìn)行，提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn)。若不能馬上進(jìn)行試驗(yàn)，可將標(biāo)本放于-20℃保存，但應(yīng)避免反復(fù)凍融
2．不能檢測含NaN3的樣品，因NaN3抑制辣根過氧化物酶的（HRP）活性。
Instrument Part    9239156    Assembly, gripper, TWI
Instrument Part    9239363    Inlet-micro-fuse T 3.15A, 250V, EZ1
Consumable    132519    NeXtal Stock Ammonium sulfate (50)    3.5M Ammonium sulfate - 50mL (4 x 12,5 ml tube)
Consumable    132554    NeXtal Stock Imidazole HCl (50)    1M Imidazole HCl - 50mL (4 x 12,5 ml tube)
Consumable    132629    NeXtal Stock Sodium citrate untit. (50)    1M tri-Sodium citrate dihydrate - Untitrated - 50mL (4 x 12,5 ml tube)
Instrument Part    9019578    Cable, Earthlink 85 mm, Cxx
Instrument Part    9019591    O-ring, sink separator, CX1
Instrument Part    9019593    Belt, Z-axis gearbox (grey), 8 ch., Cx8
Instrument Part    9019622    Cable, P assembly, 1ch., Cx2
Consumable    9019063    PyroMark Q24 MDx Software    For methylation and mutation analysis using Pyrosequencing technology in 24 well CE-marked.
Consumable    979105    PyroMark Q96 HS Tip Storage Box
Consumable    979202    PyroMark Q24 Cartridge (3)    For delivery of nucleotides and reagents for use in PyroMark Q24.
Instrument Part    9019459    Transformer (115V), HS
Instrument Part    981103    Strip Tubes and Caps, 0.1 ml (250)
Consumable    335452    EpiTect? Methyl II DNA Restriction Kit
Instrument Part    9017840    Tool, calibration tip, QIAC
Consumable    69504    DNeasy Blood & Tissue Kit (50)    50 DNeasy Spin Columns, QIAGEN Proteinase K, Buffers, Collection Tubes (2 ml)
Consumable    74126    RNeasy Protect Mini Kit (250)    RNAlater RNA Stabilization Reagent (250 ml), 250 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers
Consumable    201225    Taq PCR Core Kit (1000)    "4 x 250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2, dNTP Mix†

NEW! Now with 10x CoralLoad PCR Buffer
Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity and minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye and a red dye, that facilitate estimation of DNA migration distance and optimization of agarose gel run time."
Consumable    301427    Effectene Transfection Reagent (4x1ml)    4 x 1 ml Effectene Reagent, Enhancer, Buffer; for 160 transfections in 60 mm dishes or 640 transfections in 12-well plates
Consumable    962141    QIAprep 96 Turbo BioRobot Kit (4)    For 4 x 96 high-purity plasmid minipreps, 4 each: TurboFilter 96 and QIAprep 96 Plates; Flat-Bottom Blocks and Lids, Reagents, Buffers, Collection Microtubes (1.2 ml) and Caps, 96-Well Microplates RB and Lids, Tape Pads
Consumable    132510    NeXtal Stock ADA untit. (50)    0.5M ADA - Untitrated - 50mL (4 x 12,5 ml tube)
Consumable    132528    NeXtal Stock CAPSO untit. (50)    0.5M CAPSO - Untitrated - 50mL (4 x 12,5 ml tube)
Consumable    132578    NeXtal Stock PEG 2,000 DME (50)    50% w/v Polyethylene glycol 2,000 dimethyl ether - 50mL (4 x 12,5 ml tube)
Consumable    132635    NeXtal Stock Zinc chloride (50)    0,1M Zinc chloride - 50mL (4 x 12,5 ml tube)
Instrument Part    9019572    Pulley, Idler X-axis, Cxx
Instrument Part    9019600    O-ring, filter housing seal, 2mm, CX1
Instrument Part    9019615    Pipettor, spring, Cx2
Instrument Part    9019618    Motor, Z-axis, Cx2
    9019663    PyroMark Vac Prep Tool, PyroQ96VW
Consumable    929706    QX 0.2 ml 12-Tube Strip Caps (80)
Consumable    972442    PyroMark Q96 SNP HFE (96)    For detection of variants in HFE gene
Consumable    972807    PyroMark Gold Q96 Reagents (50 x 96)    For performing pyrosequncing reaction on the Q96 MD
Consumable    979013    Fungi ITS2 Primer 1 for Sequencing
Instrument Part    9019453    Front label incl. cutout for  P/R, HS
Consumable    990604    Elution Plate 8-Strip Caps (120)
Instrument Part    981106    Strip Tubes and Caps, 0.1 ml (2500)
Instrument Part    981604    Rotor-Disc Heat Sealing Film (600)
Consumable    950207    Reagent Pack, VX    The Reagent Pack, VX, which is used together with the Virus Plasticware Pack, QIAxtractor (#950047) provides fully automated viral nucleic acid purification on the QIAxtractor. The combination of reagents and plasticware enables purification of viral nucleic acids from liquid samples, with a throughput of 8-96 samples per run.
Consumable    288115    bactotype Mg/Ms PCR Reagent (96)
Instrument Part    9022655    Riser Block EMTR, QXT
Consumable    10091    QIAGEN-tip 10000 (5)    5 columns; 10 mg column capacity
Consumable    51304    QIAamp DNA Mini Kit (50)    For 50 DNA preps: 50 QIAamp Mini Spin Columns, QIAGEN Proteinase K, Reagents, Buffers, Collection Tubes (2 ml)
Consumable    201203    Taq DNA Polymerase (250)    "250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2
NEW! Now with 10x CoralLoad PCR Buffer

Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity and minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye and a red dye, that facilitate estimation of DNA migration distance and optimization of agarose gel run time."
Accessory    19571    AirPore Tape Sheets (50)    Microporous tape sheets for covering 96-well blocks: 50 sheets per pack
Consumable    19763    QIAfilter Maxi Cartridges (25)    25 QIAfilter Maxi Cartridges
大鼠L選擇素(L-Selectin/CD62L)ELISA試劑盒
大鼠單核細(xì)胞趨化蛋白1(MCP-1/CCL2/MCAF)ELISA試劑盒
大鼠單核細(xì)胞趨化蛋白2(MCP-2/CCL8)ELISA試劑盒
大鼠單核細(xì)胞趨化蛋白3(MCP-3/CCL7)ELISA試劑盒
大鼠巨噬細(xì)胞集落刺激因子(M-CSF)ELISA試劑盒
大鼠巨噬細(xì)胞來源的趨化因子(MDC/CCL22)ELISA試劑盒
大鼠巨噬細(xì)胞炎性蛋白2(MIP-2)ELISA試劑盒
大鼠巨噬細(xì)胞炎性蛋白3β(MIP-3β/ELC/CCL19)ELISA試劑盒
大鼠基質(zhì)金屬蛋白酶1(MMP-1)ELISA試劑盒
大鼠基質(zhì)金屬蛋白酶10(MMP-10)ELISA試劑盒
大鼠基質(zhì)金屬蛋白酶13(MMP-13)ELISA試劑盒
大鼠基質(zhì)金屬蛋白酶2/明膠酶A(MMP-2/GelatinaseA)ELISA試劑盒
大鼠基質(zhì)金屬蛋白酶3(MMP-3)ELISA試劑盒
大鼠基質(zhì)金屬蛋白酶7(MMP-7)ELISA試劑盒
大鼠基質(zhì)金屬蛋白酶8/中性粒細(xì)胞膠原酶(MMP-8)ELISA試劑盒
大鼠5核苷酸酶(5-NT)ELISA試劑盒
大鼠骨保護(hù)素(OPG)ELISA試劑盒
大鼠B因子(BF)ELISA試劑盒
大鼠抑瘤素M(OSM)ELISA試劑盒
大鼠胎盤生長因子(PLGF)ELISA試劑盒
大鼠正常T細(xì)胞表達(dá)和分泌因子(RANTES/CCL5)ELISA試劑盒
大鼠L苯*解氨酶(PAL)ELISA試劑盒
大鼠可溶性CD30配體(sCD30L)ELISA試劑盒
大鼠可溶性CD40配體(sCD40L)ELISA試劑盒
大鼠干細(xì)胞因子受體(SCFR)ELISA試劑盒
大鼠基質(zhì)細(xì)胞衍生因子1β(SDF-1β/CXCL12)ELISA試劑盒
大鼠血管生成素受體Tie1(ANG-R-Tie1)ELISA試劑盒
大鼠含免疫球蛋白樣環(huán)和上皮生長因子樣域*激酶2(Tie-2)ELISA試劑盒
大鼠基質(zhì)金屬蛋白酶抑制因子2(TIMP-2)ELISA試劑盒
大鼠基質(zhì)金屬蛋白酶抑制因子3(TIMP-3)ELISA試劑盒
大鼠基質(zhì)金屬蛋白酶抑制因子4(TIMP-4)ELISA試劑盒
大鼠腫瘤壞死因子可溶性受體Ⅰ(TNFsR-Ⅰ)ELISA試劑盒
大鼠腫瘤壞死因子可溶性受體Ⅱ(TNFsR-Ⅱ)ELISA試劑盒
大鼠腫瘤壞死因子β(TNF-β)ELISA試劑盒
小鼠L選擇素(L-Selectin/CD62L)ELISA試劑盒
大鼠腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體1(TRAIL-R1)ELISA試劑盒