人去唾液酸糖蛋白受體(AGSPR)ELISA檢測(cè)試劑盒英文名稱Human asialoglyco protein receptor,AGSPR ELISA試劑盒齊一生物科技有限公司主要經(jīng)營(yíng)進(jìn)口原裝Elisa試劑盒,種類齊全,價(jià)格實(shí)惠,貨期短.購(gòu)買我公司產(chǎn)品新老客戶,可享全程技術(shù)指導(dǎo)：(包括售前標(biāo)本收集、后期實(shí)驗(yàn)、實(shí)驗(yàn)結(jié)束后數(shù)據(jù)分析等技術(shù)問題

人去唾液酸糖蛋白受體(AGSPR)ELISA檢測(cè)試劑盒產(chǎn)品規(guī)格：96T/48T
產(chǎn)品庫(kù)存：現(xiàn)貨
產(chǎn)品價(jià)格：詢價(jià)
使用范圍：僅供科研檢測(cè),不得用于臨床.
保存條件：2-8℃
發(fā)貨方式：專門快遞免費(fèi)送貨上門
產(chǎn)品價(jià)格：*,洽談！
說(shuō)明書:說(shuō)明書隨貨發(fā)送,您也可以直接我司在線銷售人員索取.全國(guó)：代理多種品牌進(jìn)口原裝、分裝“ELISA試劑盒".齊一生物科技有限公司作為酶聯(lián)免疫供應(yīng)商,憑借*的技術(shù),*的售前,售中,售后免費(fèi)代測(cè)服務(wù),共同鑄就高品質(zhì)的產(chǎn)品.多年專業(yè)酶免服務(wù),質(zhì)量保證,可免費(fèi)提供代測(cè)服務(wù),一周內(nèi)出結(jié)果.咨詢訂購(gòu).
ELISA試劑盒操作步驟
ELISA試劑盒使用前,將所有試劑充分混勻.不要使液體產(chǎn)生大量的泡沫,以免加樣時(shí)加入大量的氣泡,產(chǎn)生加樣上的誤差.
根據(jù)待測(cè)樣品數(shù)量加上標(biāo)準(zhǔn)品的數(shù)量決定所需的板條數(shù).每個(gè)標(biāo)準(zhǔn)品和空白孔建議做復(fù)孔.每個(gè)樣品根據(jù)自己的數(shù)量來(lái)定,能使用復(fù)孔的盡量做復(fù)孔.標(biāo)本用標(biāo)本稀釋液1：1稀釋后加入50ul于反應(yīng)孔內(nèi).
加入稀釋好后的標(biāo)準(zhǔn)品50ul于反應(yīng)孔、加入待測(cè)樣品50ul于反應(yīng)孔內(nèi).立即加入50ul的*標(biāo)記的抗體.蓋上膜板,輕輕振蕩混勻,37℃溫育1小時(shí).
甩去孔內(nèi)液體,每孔加滿洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干.重復(fù)此操作3次.如果用洗板機(jī)洗滌,洗滌次數(shù)增加一次.
每孔加入80ul的親和鏈酶素-HRP,輕輕振蕩混勻,37℃溫育30分鐘.
甩去孔內(nèi)液體,每孔加滿洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干.重復(fù)此操作3次.如果用洗板機(jī)洗滌,洗滌次數(shù)增加一次.
每孔加入底物A、B各50ul,輕輕振蕩混勻,37℃溫育10分鐘.避免光照.
取出酶標(biāo)板,迅速加入50ul終止液,加入終止液后應(yīng)立即測(cè)定結(jié)果.
在450nm波長(zhǎng)處測(cè)定各孔的OD值.
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130701    NeXtal Tubes Classics Suite        8,780.00
130712    NeXtal Tubes MBClass II Suite        8,780.00
990390    Reagent Bottle Rack        339.00
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9019877    Cover, main, blue, RG6        4,120.00
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120018    QIAquick Buffer Module 8000        6,270.00
203203    HotStarTaq DNA Polymerase (250)     - 250 units HotStarTaq DNA 人去唾液酸糖蛋白受體(AGSPR)ELISA檢測(cè)試劑盒Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2    1,810.00
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