人多巴胺D2受體(D2R)ELISA試劑盒Human dopamine D2 receptor,D2R ELISA試劑盒齊一生物科技（上海）有限公司以真誠、主動、周到、規(guī)范、熱情的服務很快得到廣大客戶的*好評.公司秉承“適應多樣化的需求,提供專業(yè)化的服務"的經(jīng)營理念,為客戶提供*的服務.歡迎社會各界朋友前來洽談業(yè)務,共創(chuàng)輝煌

人多巴胺D2受體(D2R)ELISA試劑盒操作步驟：
1、標準品的稀釋：在酶標包被板上設(shè)標準品孔10孔，在*、第二孔中分別加標準品100μl，然后在*、第二孔中加標準品稀釋液50μl，混勻；然后從*孔、第二孔中各取100μl分別加到第三孔和第四孔，再在第三、第四孔分別加標準品稀釋液50μl，混勻；然后在第三孔和第四孔中先各取50μl棄掉，再各取50μl分別加到第五、第六孔中，再在第五、第六孔中分別加標準品稀釋液50ul，混勻；混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中，再在第七、第八孔中分別加標準品稀釋液50μl，混勻后從第七、第八孔中分別取50μl加到第九、第十孔中，再在第九第十孔分別加標準品稀釋液50μl，混勻后從第九第十孔中各取50μl棄掉。（稀釋后各孔加樣量都為50μl，濃度分別為9mmol/L，6 mmol/L，3 mmol/L，1.5mmol/L， 0.75 mmol/L）。
2、加樣：分別設(shè)空白孔（空白對照孔不加樣品及酶標試劑，其余各步操作相同）、待測樣品孔。在酶標包被板上待測樣品孔中先加樣品稀釋液40μl，然后再加待測樣品10μl（樣品zui終稀釋度為5倍）。加樣將樣品加于酶標板孔底部，盡量不觸及孔壁，輕輕晃動混勻。
3、溫育：用封板膜封板后置37℃溫育30分鐘。
4、配液：將30（48T的20倍）倍濃縮洗滌液用蒸餾水30（48T的20倍）倍稀釋后備用。
5、洗滌：小心揭掉封板膜，棄去液體，甩干，每孔加滿洗滌液，靜置30秒后棄去，如此重復5次，拍干。
6、加酶：每孔加入酶標試劑50μl，空白孔除外。
7、溫育：操作同3。
8、洗滌：操作同5。
9、顯色：每孔先加入顯色劑A50μl，再加入顯色劑B50μl，輕輕震蕩混勻，37℃避光顯色15分鐘. 
10、終止：每孔加終止液50μl，終止反應（此時藍色立轉(zhuǎn)黃色）。
11、測定：以空白空調(diào)零，450nm波長依序測量各孔的吸光度（OD值）。 測定應在加終止液后15分鐘以內(nèi)進行。
Guinea Pig T3 ELISA Kit   
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人多巴胺D2受體(D2R)ELISA試劑盒Guinea Pig THAA ELISA Kit  
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Guinea Pig TP ELISA Kit   
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Guinea Pig TSHR ELISA Kit 
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Guinea Pig TX-A2 ELISA Kit   
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Guinea Pig Very Low Density Lipoprotein Receptor (VLDLR) ELISA Kit
Guinea Pig Vanillylmandelic Acid (VMA)  ELISA Kit 
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31226    Pierce(R) Goat Anti-Rat IgG Fc,  2 MG    2 MG
31232    ImmunoPure (R) Goat Anti-Mouse IgG Fc gamma fragment    1 MG
31234    Pierce(R) Goat Anti-Rabbit IgG {F(ab')2}    2.0 MG
31235    Pierce(R) Rabbit IgG, Whole   Molecule, 10 mg    10 MG
31236    ImmunoPure (R) Goat Anti-Mouse IgG Fc gamma Subclass 1 (min    1 MG
31237    ImmunoPure (R) Goat Anti-Mouse IgG Fc gamma Subclass 2a (min    1 MG
31238    ImmunoPure (R) Donkey Anti-Rabbit IgG (H+L) (min x BvChGtGuH    1 MG
31239    ImmunoPure (R) F(ab')2        Fragment Goat Anti-Rabbit IgG    1 MG
31240    Pierce(R) Rabbit Anti-Sheep IgG (H+L), 2 MG    2 MG
31242    ImmunoPure (R) Mouse          Monoclonal Anti-Fluorescein    1 MG
31243    Pierce(R) Sheep IgG, Whole Molecule, 10 mg    10 MG
31245    Goat IgG, Whole Molecule,     10 mg.    10 MG
31246    Hamster IgG, Whole Molecule,  5 mg.    5 MG
31300    Pierce(R) Phosphatase Conjugated Rabbit Anti-Goat IgG (H+L),    1 ML
31302    Pierce(R) Phosphatase Conjugated F(ab')2 Fragment Rabbit Ant    0.5 ML
31310    Pierce(R) Phosphatase Conjugated Goat Anti-Human IgG (H+L),    1 ML
31312    Pierce(R) Phosphatase Conjugated Goat Anti-Human IgG F(ab')    1 ML
31314    Pierce(R) Phosphatase Conjugated Goat Anti-Human IgAa, 1 mL    1 ML
31316    Pierce(R) Phosphatase Conjugated Goat Anti-Human IgA+IgG+IgM    1 ML
31318    Pierce(R) Phosphatase Conjugated Rabbit Anti-Human IgG (Fc)    1.0 ML
31320    Pierce(R) Goat Anti-Mouse IgG,(H&L), Alkaline Phosphatase    1 ML
31321    Pierce Goat Anti-Mouse IgG    (H+L) Alkaline Phosphatase    0.2 MG
31322    Pierce(R) Phosphatase Conjugated Goat Anti-Mouse IgG (H+L),    1 ML
31324    Pierce(R) Phosphatase Conjugated Goat Anti-Mouse IgG F(ab')    1 ML
31325    Pierce(R) Phosphatase Conjugated Goat Anti-Mouse IgG (Fc)    1.0 ML
31326    Pierce(R) Phosphatase Conjugated Goat Anti-Mouse IgMu, 1 mL    1 ML
31327    Pierce(R) Phosphatase Conjugated Goat Anti-Mouse IgG (Fc)    1.0 ML
31328    Pierce(R) Phosphtase Conjugated Goat Anti-Mouse IgG + IgM    1 ML
31329    Pierce(R) Phosphatase Conjuga ted Rabbit Anti-Mouse IgG (H +    1.0 ML
31330    Pierce(R) Phosphatase Conjugated Goat Anti-Mouse IgG+IgM (H    1 ML
31331    Pierce(R) Phosphatase Conjugated Rabbit Anti-Mouse IgG {F(a    1.0 ML
31332    Pierce(R) Phosphatase Conjugated Rabbit Anti-Mouse IgG (Fc)    1.0 ML
31333    Pierce(R) Phosphatase Conjugated Rabbit Anti-Mouse IgM (u)    1.0 ML
31334    Pierce(R) Phosphatase Conjugated Rabbit Anti-Mouse IgG (H+L    0.5 ML
31335    Pierce(R) Phosphatase Conjugated Rabbit Anti-Mouse IgG + Ig    1.0 ML
31337    Pierce(R) Phosphatase Conjugated Rabbit Anti-Goat IgG (Fc)    1.0 ML
31340    Pierce(R) Goat Anti-Rabbit IgG, (H+L), Alkaline Phosphatase    1 ML
31341    ImmunoPure Phosphatase Conjugated Goat Anti-Rabbit IgG (Fc)    1.0 ML
31342    Pierce(R) Phosphatase Conjuga ted Goat ANti-Rabbit IgG (H+L)    1 ML
31343    Pierce(R) Phosphatase Conjugated Goat Anti-Rabbit IgG    1.0 ML
31345    ImmunoPure Phosphatase Conj. Donkey Anti-Rabbit IgG(H+L)(min    0.5 ML
31346    Pierce Goat Anti-Rabbit IgG   (H+L) Alkaline Phosphatase    0.2 MG
31350    Pierce(R) Phosphatase Conjugated Goat Anti-RatIgG (H+L), 1    1 ML
31360    Pierce(R) Phosphatase Conjugated Rabbit Anti-Sheep IgG (H+L)    1 ML
31391     Alkaline Phosphatase      20 mg 
31392     Alkaline Phosphatase      100 mg 
31400    Pierce(R) Peroxidase Conjugated Mouse Anti-Goat IgG (H+L)    1 ML
31401    Pierce(R) Peroxidase Conjugat ed Rabbit Anti-Chicken IgY (H+    1.5 ML
31402    Pierce(R) Peroxidase Conjugated Rabbit Anti-Goat IgG (H+L),    1.5 ML
31403    Pierce(R) Peroxidase Conjugated Rabbit Anti-Goat IgG {F(ab'    1.5 ML
31410    Pierce(R) Peroxidase Conjugated Goat Anti-Human IgG (H+L),    2 ML
31412    Pierce(R) Peroxidase Conjugated Goat Anti-Human IgG (H+L)(m    1.5 ML
31413    Pierce(R) Peroxidase Conjugated Goat Anti-Human IgG FC    1 ML
31414    Pierce(R) Peroxidase Conjugated Goat Anti-Human IgG F(ab')2    1.5 ML
31415    Pierce(R) Peroxidase Conjugated Goat Anti-Human IgM (Fc5u)    2 ML
31417    Pierce(R) Peroxidase Conjugated Goat Anti-Human IgAa, 2 ML    2 ML
31418    Pierce(R) Peroxidase Conjugated Goat Anti-Human Immunoglobul    2 ML
31420    Pierce(R) Peroxidase Conjugated Mouse Anti-Human IgG)H+L)(m    1.5 ML
31423    Pierce(R) Peroxidase Conjugated Rabbit Anti-Human IgG (Fc)    1.5 ML
31430    Pierce(R) Goat Anti-Mouse IgG,(H&L), Peroxidase Conjugated,    2 ML
31431    ImmunoPure Peroxidase         Conjugated Goat Anti-Mouse    0.2 ML
31432    Pierce(R) Peroxidase Conjugated Goat Anti-Mouse IgG (H+L)    1.5 ML
31433    Pierce(R) Peroxidase Conjugat ed Rabbit Anti-Goat IgG (Fc)    1.5 ML
31436    Pierce(R) Peroxidase Conjugated Goat Anti-Mouse IgG F(ab')2    2 ML
31437    Pierce(R) Peroxidase Conjugated Goat Anti-Mouse IgG (Fc)    2.0 ML
31438    Pierce(R) Peroxidase Conjugated F(ab')2 Fragment Goat Anit-    0.5 ML
31439    Pierce(R) Peroxidase Conjugated Goat Anti-Mouse IgG (Fc) (mi    1.5 ML
31440    Pierce(R) Peroxidase Conjugated Goat Anti-Mouse IgMu, 2 ML    2 ML
31444    Pierce(R) Peroxidase Conjugated Goat Anti-Mouse IgG+IgM (H+    2 ML
31446    Pierce(R) Peroxidase Conjugated Goat Anti-Mouse IgG+IgM (H+L    1.5 ML
31448    Pierce(R) Peroxidase Conjugated F(ab')2 Fragment Goat Anti-    0.5 ML
31450    Pierce(R) Peroxidase Conjugated Rabbit Anti-Mouse IgG (H+L)    1.5 ML
31451    Pierce(R) Peroxidase Conjugated Rabbit Anti-Mouse IgG {F(ab    1.5 ML