人心鈉肽(ANP)ELISA試劑盒英文名稱Human Atrial Natriuretic Peptide,ANP ELISA試劑盒齊一生物科技（上海）有限公司以真誠(chéng)、主動(dòng)、周到、規(guī)范、熱情的服務(wù)很快得到廣大客戶的*好評(píng).公司秉承“適應(yīng)多樣化的需求,提供專業(yè)化的服務(wù)"的經(jīng)營(yíng)理念,為客戶提供*的服務(wù).歡迎社會(huì)各界朋友前來(lái)洽談業(yè)務(wù),共創(chuàng)輝煌

人心鈉肽(ANP)ELISA試劑盒操作步驟：
1、標(biāo)準(zhǔn)品的稀釋：在酶標(biāo)包被板上設(shè)標(biāo)準(zhǔn)品孔10孔，在*、第二孔中分別加標(biāo)準(zhǔn)品100μl，然后在*、第二孔中加標(biāo)準(zhǔn)品稀釋液50μl，混勻；然后從*孔、第二孔中各取100μl分別加到第三孔和第四孔，再在第三、第四孔分別加標(biāo)準(zhǔn)品稀釋液50μl，混勻；然后在第三孔和第四孔中先各取50μl棄掉，再各取50μl分別加到第五、第六孔中，再在第五、第六孔中分別加標(biāo)準(zhǔn)品稀釋液50ul，混勻；混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中，再在第七、第八孔中分別加標(biāo)準(zhǔn)品稀釋液50μl，混勻后從第七、第八孔中分別取50μl加到第九、第十孔中，再在第九第十孔分別加標(biāo)準(zhǔn)品稀釋液50μl，混勻后從第九第十孔中各取50μl棄掉。（稀釋后各孔加樣量都為50μl，濃度分別為9mmol/L，6 mmol/L，3 mmol/L，1.5mmol/L， 0.75 mmol/L）。
2、加樣：分別設(shè)空白孔（空白對(duì)照孔不加樣品及酶標(biāo)試劑，其余各步操作相同）、待測(cè)樣品孔。在酶標(biāo)包被板上待測(cè)樣品孔中先加樣品稀釋液40μl，然后再加待測(cè)樣品10μl（樣品zui終稀釋度為5倍）。加樣將樣品加于酶標(biāo)板孔底部，盡量不觸及孔壁，輕輕晃動(dòng)混勻。
3、溫育：用封板膜封板后置37℃溫育30分鐘。
4、配液：將30（48T的20倍）倍濃縮洗滌液用蒸餾水30（48T的20倍）倍稀釋后備用。
5、洗滌：小心揭掉封板膜，棄去液體，甩干，每孔加滿洗滌液，靜置30秒后棄去，如此重復(fù)5次，拍干。
6、加酶：每孔加入酶標(biāo)試劑50μl，空白孔除外。
7、溫育：操作同3。
8、洗滌：操作同5。
9、顯色：每孔先加入顯色劑A50μl，再加入顯色劑B50μl，輕輕震蕩混勻，37℃避光顯色15分鐘. 
10、終止：每孔加終止液50μl，終止反應(yīng)（此時(shí)藍(lán)色立轉(zhuǎn)黃色）。
11、測(cè)定：以空白空調(diào)零，450nm波長(zhǎng)依序測(cè)量各孔的吸光度（OD值）。 測(cè)定應(yīng)在加終止液后15分鐘以內(nèi)進(jìn)行。
31030     High Sensitivity NeutrAvidin-HRP      0.5 mL 
31032     High Sensitivity NeutrAvidin-HRP      5 mL 
31050     NeutrAvidin Protein      100 mg 
31055     NeutrAvidin Protein      1000 mg 
31104    Pierce(R) Rabbit Anti-Chicken IgY (H+L)    2.0 MG
31105    Pierce(R) Rabbit Anti-Goat IgG (H+L), 2 MG    2 MG
31107    Pierce(R) Mouse Anti-Goat IgG (H+L) (min x HnMsRb Sr Prot)    1.5 MG
31109    Pierce(R) F(ab')2 Fragment    Rabbit Anti-Goat IgG (H+L) (mi    0.5 MG
31111     Immunodiffusion Plates, Agarose Gelling Agent, Multiple Pattern      6/PKG 
31115    Pierce(R) Goat Anti-Hamster   IgG (H+L)    1.5 MG
31118    Pierce(R) Goat Anti-Human IgG  (Gamma)    0.5 MG
31119    Pierce(R) Goat Anti-Human     IgG (H+L), (min x BvHsMs Sr    1.5 MG
31122    Pierce(R) Goat Anti-Human     IgG {F(ab')2}    2.0 MG
31124    Pierce(R) Goat Anti-Human IgM (u)    0.5 MG
31125    ImmunoPure Goat Anti-Human    IgG (Fc, (min x BvHsMs Sr Prot    1 MG
31128    Pierce(R) Goat Anti-Human     IgA + IgG + IgM (H+L)    2.0 MG
31129    Pierce(R) Goat Anti-Human Kappa Chain    0.5 MG
31130    Pierce(R) Goat Anti-Human IgG (H+L), 2 mg    2 MG
31131    Pierce(R) Goat Anti-Human Lambda Chain    0.5 MG
31132    Pierce(R) Goat Anti-Human IgG  F(ab')2, (min X Bv Hs Ms Sr P    1.5 MG
31133    Pierce(R) Rabbit Anti-Goat IgG (Fc)    2.0 MG
31135    Pierce(R) Mouse Anti-Human IgG (H+L), (min x Ms Sr Prot)    2.0 MG
31136    Pierce(R) GOAT ANTI-HUMAN IgM  Fc5u, 2 MG    2 MG
31137    Pierce(R) Mouse Anti-Human    IgG (H+L), (min x BvHsMs Sr    1.5 MG
31140    Pierce(R) Goat Anti-Human Serum IgAa, 2 MG    2 MG
31142    Pierce(R) Rabbit Anti-Human   IgG Fc, 2 MG    2 MG
31143    Pierce(R) Rabbit Anti-Human   IgG (H+L)    2.0 MG
31146    Pierce(R) Human IgM (Myeloma), Whole Molecule, 2.0 mg    2 MG
31148    Pierce(R) Human IgA Serum,    2.0 mg    2 MG
31154    Pierce(R) Human IgG, Whole    Molecule, 10 mg.    10 MG
31160    Pierce(R) Goat Anti-Mouse IgG (H+L), 2 mg    2 MG
31163    Pierce(R) F(ab')2 Fragment    Goat Anti-Human IgG (Fc)    1.0 MG
31164    Pierce(R) GOAT ANTI-MOUSE IgG  (H+L)(min X HnBvHs Sr PROT),    1.5 MG
31165    Pierce(R) F(ab')2 Fragment    Goat Anti-Human IgG (H+L)    1.0 MG
31166    Pierce(R) Goat Anti-Mouse IgG F(ab')2, 2 mg    2 MG
31168    Pierce(R) Goat Anti-Mouse IgG Fc, 2 mg    2 MG
31170    Pierce(R) Goat Anti-Mouse IgG Fc (min X HnBvHs Sr PROT), 1.5    1.5 MG
31172    Pierce(R) GOAT ANTI-MOUSE IgM u, 2 MG    2 MG
31178    Pierce(R) F(ab')2 FRAGMENT GO AT ANTI-MOUSE IgMu, 1 MG    1 MG
31181    Pierce(R) Horse Anti-Mouse Ig G (H+L)    1.5 MG
31182    Pierce(R) GOAT ANTI-MOUSE IgG +IgM (H+L), 2 MG    2 MG
31185    Pierce(R) F(ab')2 Fragment    Goat Anti-Mouse IgG (H+L)    1.0 MG
31186    Pierce(R) F(ab')2 Fragment    Goat Anti-Mouse IgM (u)    1.0 MG
31188    Pierce(R) Rabbit Anti-Mouse,  IgG (H+L), 2 mg    2 MG
31190    Pierce(R) Rabbit Anti-Mouse   IgG(H+L)(min X Hn Sr PROT),    1.5 MG
31192    Pierce(R) Rabbit Anti-Mouse IgG F(ab')2, 2 MG    2 MG
31194    Pierce(R) Rabbit Anti-Mouse   IgG Fc, 2 mg    2 MG
31196    Pierce(R) Rabbit Anti-Mouse IgMu, 2 MG    2 MG
31198    Pierce(R) Rabbit Anti-Mouse IgG+IgM (H+L), 2 MG    2 MG
31203    Pierce(R) Mouse IgG, F(ab')2  Fragment    2 MG
31205    Pierce(R) Mouse IgG, FC Fragm ent    1 MG
31210    Pierce(R) Goat Anti-Rabbit    IgG  (H+L), 2 mg    2 MG
31212    Pierce(R) Goat Anti-Rabbit IgG (H+L)(min X Hn Sr PROT), 1.5    1.5 MG
31213    Pierce(R) Mouse Anti-Rabbit   IgG (H+L), (min x GtHnMsSh Sr    1.5 MG
31216    Pierce(R) Goat Anti-Rabbit IgG Fc, 2 MG    2 MG
31218    Pierce(R) Rabbit Anti-Rat     IgG (H+L)    2.0 MG
31219    Pierce(R) Rabbit Anti-Rat IgG  (H+L), (min x Ms Sr Prot)    0.5 MG
52906    QIAamp Viral RNA Mini Kit (250)    For 250 RNA preps: 250 QIAamp Mini Spin Columns, Carrier RNA, Collection Tubes (2 ml), RNase-free Buffers
63183    DyeEx 96 Kit (24)    24 DyeEx 96 Plates; 4 Collection Plates, 48-Well
69582    DNeasy 96 Blood & Tissue Kit (12)    For 12 x 96 DNA minipreps: 12 DNeasy 96 Plates, QIAGEN Proteinase K, Buffers, Square-Well Blocks, AirPore Tape Sheets, Collection Microtubes (1.2 ml), Caps, 96-well Plate Registers
70042    Oligotex mRNA Midi Kit    For 12 mRNA midipreps: 700 ul Oligotex Suspension, Small Spin Columns, Collection Tubes (1.5 ml), RNase-free Reagents and Buffers
79523    Small Spin Columns    24 RNase-free spin columns for Oligotex spin procedures
301109    PolyFect Transfection Reagent (10x100ml)
9239361    PCB-micro-fuse T 6.3A, 250V, EZ1
人心鈉肽(ANP)ELISA試劑盒132632    NeXtal Stock Potassium formate (50)    10M Potassium formate - 50mL (4 x 12,5 ml tube)
9019610    Cover, Pipettor, 1 ch. (white), CAS
9019624    PCB, lge breakout, 12 core to rev 3, Cx2
9019067    PyroMark CpG SW 1.0    Software for PyroMark Q96 ID and PyroMark Q96 MD for analysis and quantification of CpG methylation in epigenetic studies.
9019087    PyroMark IdentiFire SW 1.0    Software for sequence-based species identification by comparing and matching Pyrosequencing data to a local database.
972052    PyroMark Q96 CpG Prader Willi (96)    For quantification of methylation level in  Prader-Willi and Angelman Syndromes
979002    PyroMark Q96 Plate Low (100)    For sample analysis of DNA template on ID.
979004    PyroMark Q96 Cartridge (3)    For delivery of nucleotides and reagents for use in PyroMark Q96 ID.
9001513    PyroMark Q24 MDx    For methylation and mutation analysis using Pyrosequencing technology in 24 well CE-marked.
990610    Filter-Tips, OnCor C, 200 μl (960)
9017842    Tool, gauge, 30 mm, calibration, QIAC
9020806    Rotor Timing Calibration Dye, RGQ
331455    miScript Microfluidics PreAMP Kit (96)
381566    Investigator ESSplex SE GO! Kit (200)
9022654    Transfer Carriage QIAplate, QXT
16191    QIAwell 96 Ultra Plasmid Kit (4)    For 4 x 96 ultrapure plasmid minipreps, 4 each: QIAfilter 96, QIAwell 96, and QIAprep 96 Plates; Reagents, Buffers, Collection Microtubes (1.2 ml), Caps
19046    Plasmid Buffer Set    Buffers P1, P2, P3, QBT, QC, QF, RNase A; for 100 plasmid mini-, 25 midi-, or 10 maxipreps
19201    Collection Tubes (2-ml)    1000 Collection Tubes (2 ml)
9015862    EZ1 DNA PRFN Card    Pre-programmed card for BioRobot EZ1 paraffin section protocols
67163    MagAttract 96 DNA Plant Core Kit (24)    MagAttract Suspension A and buffers for 24 x 96 preps
1030483    Elution Microtubes CL, PAX, FLU
9232687    Hood f. Tip Disposal Station 2.01
9015972    Tube Holder, STS MDx, 15 mm (12)
158926    Cell Suspension Solution (125 ml)
159992    Generation DNA Purif. Soln 1 (1000 ml)
201913    dNTP Set, PCR Grade 100mM, (4x250μl)    dNTP Set is a complete set of individual, highly pure dNTPs: 100 mM each dATP, dCTP, dGTP, dTTP for 2500 x 50ul PCR reactions
12945    QIAGEN Plasmid Plus Midi Kit (100)    "100 QIAGEN Plasmid Plus Midi Columns, Extender Tubes, Reagents, Buffers, 100 QIAfilter Midi Cartridges
QIAGEN Plasmid Plus Kits enable ultrafast, large-scale plasmid purification of pure plasmid DNA. The use of a vacuum manifold allows purification of up to 12 (Mega and Giga) or up to 24 (Midi and Maxi) samples in parallel with low hands-on needs. Low elution volumes yield highly concentrated plasmid DNA for direct use without ethanol precipitation. QIAGEN Plasmid Plus Kits also feature a novel wash buffer for endotoxin reduction."
9019715    PyroMark Q24 User Manual
9019861    Rotor Locking Nut Set
9019890    Foam insert, rotor securing, QIAC
336391    SureSilencing shRNA Control
330522    RT2 SYBR Green ROX qPCR Mastermix (12)
330411    RT2 HT First Strand Kit (96)
336161    Multi-Analyte  ELISArray Kit
9020716    Optoswitch, X axis, C34
9020712    Rail, x axis, C34
9242544    Power Input Module, Eppendorf, AP196/98
9020713    Rail guide, x axis, C34
9020911    Foot assembly, SMC, QGX
9020679    Rail, left, CX1
9022916    Gas press.spring conversion kit QG/QXT
9012771    Liquid Handling Kit Plato 7382/7182
9013150    BGR Tischabstützung  Digene
9013205    BGR Labhand li+re Digene