細胞名稱

BHK-21 [C-13]倉鼠腎成纖維細胞

描述

1961年3月，I.A. Macpherson和M.G.P. Stoker從1日齡的倉鼠建立了BHK-21 (C-13)的親本。隨后84天連續(xù)培養(yǎng)，僅中斷8天進行凍存，通過單細胞分離建立了13號克隆。這株細胞可用作轉染宿主，用于表達包含選擇及擴增標記的DNA(如Factor VIII,見ATCC CRL-8544)的載體。

動物種別

倉鼠

性別

***

組織來源

正常腎

形態(tài)

成纖維細胞

培養(yǎng)基和添加劑

MEM培養(yǎng)基(GIBCO,貨號41500034，添加NaHCO3 1.5g/L，Sodium Pyruvate 0.11g/L)，90%；優(yōu)質胎牛血清，10%。 氣相：空氣，95%；二氧化碳，5%。 溫度：37攝氏度。

供應限制

REFERENCE

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