細(xì)胞名稱

OKT 3 暫不供應(yīng)雜交瘤(抗CD3)

描述

用人外周血淋巴細(xì)胞免疫小鼠。取其脾細(xì)胞與P3x63Ag8.U1骨髓瘤細(xì)胞融合。

動(dòng)物種別

雜交瘤

性別

***

組織來(lái)源

細(xì)胞類型：雜交瘤；B淋巴細(xì)胞

形態(tài)

淋巴母細(xì)胞

培養(yǎng)基和添加劑

IMDM培養(yǎng)基(GIBCO,貨號(hào)12200036)，80%；優(yōu)質(zhì)胎牛血清，20%。 氣相：空氣，95%；二氧化碳，5%。 溫度：37攝氏度。

供應(yīng)限制

REFERENCE

Hoffman RA, et al. Simple and rapid measurement of human T lymphocytes and their subclasses in peripheral blood. Proc. Natl. Acad. Sci. USA 77: 4914-4917, 1980. PubMed: 6968909 Kung PC, Goldstein G. Complement-fixing monoclonal antibody to human T cells, and methods of preparing same. US Patent 4,361,549 dated Nov 30 1982 Tanaka Y, et al. Productive and lytic infection of human CD4+ type 1 helper T cells with macrophage-tropic human immunodeficiency virus type 1. J. Virol. 71: 465-470, 1997. PubMed: 8985372 Solomon KR, et al. The association between glycoslphosphatidylinositol-anchored proteins and hetrotrimeric G protein alpha subunits in lymphocytes. Proc. Natl. Acad. Sci. USA 93: 6053-6058, 1996. PubMed: 8650218 Hutchcroft JE, et al. Differential phosphorylation of the T lymphocyte costimulatory receptor CD28. J. Biol. Chem. 271: 13362-13370, 1996. PubMed: 8662792 Hartley D, Corvera S. Formation of c-Cb1-phosphatidylinositol 3-kinase complexes on lymphocyte membranes by a p56lck-independent mechanism. J. Biol. Chem. 271: 21939-21943, 1996. PubMed: 8702998