細(xì)胞名稱

MDA-MB-435S人黑素瘤細(xì)胞

描述

MDA-MB-435S是一種紡錘形的細(xì)胞，1976年由其親本(435)中篩選得到。435是從31歲的轉(zhuǎn)移性乳腺導(dǎo)管腺癌女性患者胸水中分離得到。 當(dāng)用熒光染料對微管蛋白進(jìn)行染色時親本細(xì)胞顯現(xiàn)散布特征(II型)。 zui近通過cDNA陣列研究表明，親本(MDA-MB-435)可歸入黑素瘤起源。

動物種別

人

性別

女

組織來源

黑素細(xì)胞，黑素瘤，以前認(rèn)為是乳腺; 乳房; 導(dǎo)管; 導(dǎo)管癌; 胸腺滲出液

形態(tài)

紡錘形

培養(yǎng)基和添加劑

L-15培養(yǎng)基(GIBCO,貨號41300039)和0.01mg/ml胰島素，90%；優(yōu)質(zhì)胎牛血清，10%。 氣相：空氣，*。 溫度：37攝氏度。

供應(yīng)限制

A。僅供研究之用。

REFERENCE

Brinkley BR , et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337 Siciliano MJ , et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779 Cailleau R , et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202 Sheng S , et al. Maspin acts at the cell membrane to inhibit invasion and motility of mammary and prostatic cancer cells. Proc. Natl. Acad. Sci. USA 93: 11669-11674, 1996. PubMed: 8876194 Zhu X , et al. Cell cycle-dependent modulation of omerase activity in tumor cells. Proc. Natl. Acad. Sci. USA 93: 6091-6095, 1996. PubMed: 8650224 Ross DT , et al. Systematic variation in gene expression patterns in human cancer cell lines. Nature Genetics 24: 227-235, 2000. PubMed: 10700174 Ellison G , et al. Further evidence to support the melanocytic origin of MDA-MB-435. Mol. Pathol. 55: 294-299, 2002. PubMed: 12354931