細(xì)胞名稱

BT-474 [BT474]人乳腺導(dǎo)管癌細(xì)胞

描述

E. Lasfargues 和 W.G. Coutinho從一個(gè)實(shí)心的乳腺浸潤(rùn)性導(dǎo)管癌中分離到了BT-474細(xì)胞株。

動(dòng)物種別

人

性別

女

組織來(lái)源

乳腺導(dǎo)管癌

形態(tài)

上皮細(xì)胞

培養(yǎng)基和添加劑

RPMI-1640(GIBCO,貨號(hào)31800022, 添加NaHCO3 1.5g/L, glucose 2.5g/L, Sodium Pyruvate 0.11g/L), 90%; FBS 10%。

供應(yīng)限制

REFERENCE

Lasfargues EY , et al. Isolation of two human tumor epithelial cell lines from solid breast carcinomas. J. Natl. Cancer Inst. 61: 967-978, 1978. PubMed: 212572 22660: Lasfargues EY , et al. A human breast tumor cell line (BT-474) that supports mouse mammary tumor virus replication. In Vitro 15: 723-729, 1979. PubMed: 94035 32275: Littlewood-Evans AJ , et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997. PubMed: 9393764