亚洲国产精品二区久久,日本美女后入式午夜视频在线观看,国产污视频在线观看,欧美日韩国产精品中文字幕在线观看

技術(shù)中心

大鼠甲狀旁腺素(PTH)英文說明書

2018年03月30日 09:10:52人氣:412來源:上海通蔚生物科技有限公司

資料類型doc文件資料大小53248
下載次數(shù)48資料圖片 【點(diǎn)擊查看】
上 傳 人上海通蔚生物科技有限公司 需要積分0
關(guān) 鍵 詞大鼠甲狀旁腺素(PTH)英文說明書
【資料簡介】

            FOR RESEARCH USE ONLYRat     PTH

Assay range:5 ng/L -160 ng/L   96 determinations
Purpose
This kit allows for the determination of PTH concentrations in Rat serum, cell culture supernates and other biological fluids

Principle of the assay
The kit assay Rat PTH level in the sample,use Purified Rat PTH antibody to coat microtiter plate wells, make solid-phase antibody, then add PTH to wells, Combined PTH antibody which With HRP labeled goat anti- Human become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat PTH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Materials provided with the kit
1    wash  solution    20ml×1bottle    7    Stop Solution    6ml×1 bottle
2    HRP-Conjugate reagent    6ml×1 bottle    8    Standard(320ng/L)    0.5ml×1 bottle
3    Microelisa stripplate    12well×8strips    9    Standard diluent    1.5ml×1bottle
4    Sample diluent    6ml×1 bottle    10    Instruction    1
5    Chromogen Solution A    6ml×1 bottle    11    Closure plate membrane    2
6    Chromogen Solution B    6ml×1 bottle    12    Sealed bags    1
Specimen requirements
1.extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.
2.Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.
Assay procedure
1.Dilute and add sample:Dilute Original density Standard as follow table:
160 ng/L    5 Standard    150μl Original density Standard+150μl Standard diluent
80 ng/L    4 Standard    150μl 5 Standard+150μl Standard diluent
40 ng/L    3 Standard    150μl 4 Standard+150μl Standard diluent
20 ng/L    2 Standard    150μl 3 Standard +150μl Standard diluent
10 ng/L    1 Standard    150μl 2 Standard +150μl Standard diluent
2.add sample:Set blank wells separay (blank comparison wells don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.
3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.
4.Configurate liquid: 30-fold(or 20-fold) wash solution diluted 30-fold (or 20-fold) with distilled water and reserve.
5.washing:Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.
6.add enzyme:Add HRP-Conjugate reagent 50μl to each well, except  blank well. 
7.incubate:Operation with 3.
8.washing:Operation with 5.
9.color:Add Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃
10.Stop the reaction:Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).
11.assay:take blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.
Steps description
Standard, Sample diluent


Add Standard, Sample diluent, incubate for 30 min at 37℃.


Wash 5 time,Add HRP-Conjugate reagent, incubate for 30 min at 37℃.


Wash 5 times,Add Chromogen Solution A and B, incubate for 30 min at 37℃.


Add Stopp Solution


Read absorbance at 450nm within 15 min


calculate
Calculate
Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.
Important notes
1.The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
2.washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.
3.add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 min, if the number of sample is much , recommend to use Volley .
4.if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente and multiplied by the dilution factor.(×n×5).
5.Closure plate membrane only limits the disposable use, to avoid cross-contamination.
6.The substrate evade the light preservation.
7.Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.
8.All samples, washing buffer and each kind of reject should according to infective material process.
9.Do not mix reagents with those from other lots.
Storage and validity
1.Storage:  2-8℃.
2.validity: six months.

上海通蔚生物科技有限公司作者

上一篇:簡述玻璃鋼堿洗塔

下一篇:板框廂式壓濾機(jī)的優(yōu)缺點(diǎn)有哪些?


我要投稿
  • 投稿請發(fā)送郵件至:(郵件標(biāo)題請備注“投稿”)hbzhan@vip.qq.com
  • 聯(lián)系電話0571-87759680
環(huán)保行業(yè)“互聯(lián)網(wǎng)+”服務(wù)平臺(tái)
環(huán)保在線APP

功能豐富 實(shí)時(shí)交流

環(huán)保在線小程序

訂閱獲取更多服務(wù)

微信公眾號

關(guān)注我們

抖音

環(huán)保在線網(wǎng)

抖音號:hbzhan

打開抖音 搜索頁掃一掃

視頻號

環(huán)保在線

公眾號:環(huán)保在線

打開微信掃碼關(guān)注視頻號

快手

環(huán)保在線

快手ID:2537047074

打開快手 掃一掃關(guān)注
意見反饋
美女逼男逼小穴小骚下载| 日本免费暖暖在线小视频| 欧美精品日韩精品中文字幕| 日本最新免费不卡一区二区三区| 亚洲男人的天堂2021| 91孕妇精品一区二区三区| 一区二区在线不卡| 亚洲AV无码一区二区三区天堂古| 国产老妇伦国产熟女91| 伊人久久丁香色婷婷啪啪| 一区二区在线不卡| 在线免费观看一区二区三区| 美女让我插她的骚逼| 日本欧美人一区二区三区| 欧美日韩综合在线一区| 欧美国产综合日韩一区二区| 欧美人人做人人爽人人喊| 日韩亚洲AV无码一区二区不卡| 国产午夜精品美女视频露脸| 18岁美女破处在线观看| 亚洲精品影片一区二区三区| 日本最新免费不卡一区二区三区| 操我骚逼抽插视频| 骚逼被操视频拳交| 制服丝袜国产在线第一页| 日韩一区二区三区夜色视频| 久久精品人人爽人人爽快| 想被操在线啊啊啊啊| 大男人在线无码直播| 插欧美美女逼逼逼逼| 9国产亚洲精品国产| 午夜性福福利视频一区二区三区| 好嗨哟直播看片在线观看| 日本人妻与家公的伦理片| 大吊肏子宫在线观看| 影音先锋亚洲中文综合网| 精品免费久久久久久久久| 亚洲av 又黄又爽十大| 国产亚洲一区白丝在线观看| 国产高欧美性情一线在线| 久久久国产系列丝袜熟女|