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>技術(shù)文章>Rabbit Anti-phospho-JNK1/2/3 (pThr183/pTyr185)抗體說明書
技術(shù)文章

Rabbit Anti-phospho-JNK1/2/3 (pThr183/pTyr185)抗體說明書

閱讀:354發(fā)布時間:2013-4-8

Rabbit Anti-phospho-JNK1/2/3 (pThr183/pTyr185)抗體說明書

Catalog Number: Rs-1640R

Quantity size: 0.1ml (dilute with pH 7.4 0.01 M PBS or antibody diluent )

Background:
 
JNK1 (MAPK8) is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrome c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. JNK1 is activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K4 and MAP2K7. The JNK pathway is critically involved in diabetes and levels are abnormally elevated in obesity. The cell-permeable JNK inhibitory peptide may have promise as a therapeutic agent for diabetes.

Specificity :
·anti-phospho-JNK1/2/3 (pThr183/pTyr185) is a rabbit monoclonal antibody       unconjugated

· specific for phospho-JNK1/2/3 (pThr183/pTyr185) of human, mouse, rat, goat, dog

· epitope mapping is synthetic phospho-peptide corresponding to residues surrounding (pThr183/pTyr185) of human, mouse, rat, goat, dog JNK1/2/3

· use for western blotting, elisa, immunoprecipitation and immunohistochemistry

· Protein G affinity chromatography purification, purity :>95%

· Isotype: IgG

· mol wt: 36kDa

Application :
· Western blotting 1:100-500

· Immunohistochemistry 1:100-500

· ELISA 1:500-1000

· Optimal working dilutions must be determined by the end user.

Storage:
 
Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.

Important Note:
 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Rabbit Anti-phospho-JNK1/2/3 (pThr183/pTyr185)抗體說明書相關(guān)產(chǎn)品:                                     ADORA1??腺苷A1A受體抗體
AMP deaminase 1??腺苷單磷酸脫氨酶1抗體
A2AR/Adenosine A2a receptor??腺苷A2A受體抗體
phospho-Rb/p105-Rb(Ser780)??磷酸化成視網(wǎng)膜細胞瘤抑癌蛋白抗體
AACT-Alpha1/A1ACT??α-1抗胰*抗體
AAK1??AP2關(guān)聯(lián)激酶1抗體
Cyp2-j3??細胞色素P450Ⅱj3抗體
AANAT??芳香胺N-乙?;D(zhuǎn)移酶抗體
TNF-alpha??腫瘤壞死因子-α抗體
AARS2??丙氨酰tRNA合成酶2抗體
SIRT1/sirtuin 1??沉默調(diào)節(jié)蛋白1抗體
TBX-5??轉(zhuǎn)錄因子Tbx5抗體
AAT/A1AT??α-1抗*抗體
AATK??AATK細胞凋亡關(guān)聯(lián)*激酶抗體
NMP-22??核基質(zhì)蛋白22
AATF??拮抗凋亡轉(zhuǎn)錄因子抗體
ABCA1/ABC1??腺苷三磷酸結(jié)合盒轉(zhuǎn)運體A1抗體
ABCD1/CCL22??嗜酸粒細胞趨化蛋白22抗體
ABCB5??ATP結(jié)合蛋白家族5抗體
ABCB6??ATP結(jié)合蛋白家族6抗體
ABCF1??ATP結(jié)合盒蛋白家族GCN20F家族1抗體
ABCG1??三磷酸腺苷結(jié)合盒亞家族G1抗體


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