Rabbit Anti- SOX11抗體操作說(shuō)明書(shū)FROM:ABCAM
Catalog Number : ab50194
Quantity size : 0.1ml （dilute with pH 7.4 0.01 M PBS or antibody diluent ）
Background:
All SOX proteins have a single HMG box and bind linear DNA in a sequence-specific manner, resulting in the bending of DNA through large angles. Bending causes the DNA helix to open for some distance, which may affect binding and interactions of other transcription factors. SOX1, SOX2 and SOX3 show the closest homology to SRY. They share maximum homology within the HMG domain and are expressed mainly in the developing nervous system of the mouse （Collignon et al., 1996）. These genes share significant homology outside the HMG box also and are highly conserved throughout their evolution. SOX11 appears to be important in the developing nervous system （SOX11 expression is detected from 8.5 dpc through to 13.5 dpc, with a considerable increase in expression by 13.5 dpc）. SOX11 expression is apparent in the somites,limbs,facial mesenchyme,kidney,lung of the fetal system.
pecificity :
- Anti- SOX11 is a rabbit polyclonal antibody unconjugated
specific for SOX11 of rat,mouse
– use for western blotting,elisa,immunoprecipitation and immunohistochemistry
– Protein G affinity chromatography purification, purity :>95%
– Isotype: IgG
Application : Rabbit Anti- SOX11抗體操作說(shuō)明書(shū)
– WB=1:100-500
– ELISA=1:500-1000
– IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 ，not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user..
Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Rabbit Anti- SOX11抗體操作說(shuō)明書(shū)
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