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>技術(shù)文章>Rabbit Anti- Cav-1抗體說(shuō)明書(shū)
技術(shù)文章

Rabbit Anti- Cav-1抗體說(shuō)明書(shū)

閱讀:202發(fā)布時(shí)間:2013-1-18

 Rabbit  Anti- Cav-1抗體說(shuō)明書(shū),新學(xué)期*

FROM:ABCAM
Catalog Number : Ab86711
Quantity size :   0.1ml (dilute with pH 7.4    0.01 M PBS or antibody diluent )
Background:
Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Autophagy, a process of bulk protein degradation through an autophagosomic lysosomal pathway, is important for differentiation, survival during nutrient deprivation, and normal growth control, and is often defective in tumor cells. Beclin 1 was originally isolated in a yeast two hybrid screen to identify Bcl 2 binding partners and maps to a tumor susceptibility locus on human chromosome 17q21 that is frequently monoallelically deleted in human breast, ovarian and prostate cancer. Beclin 1 encodes an evolutionarily conserved 52kDa coiled coil protein that is expressed in human muscle, epithelial cells and neurons.
.pecificity :   
-    Anti- Cav-1 is a rabbit  polyclonal antibody unconjugated                 
specific for Cav-1 of   hu, rat, mo
         use for western blotting,elisa,immunoprecipitation and immunohistochemistry
         Protein G affinity chromatography purification, purity :>95%
         Isotype:  IgG
Application :  

Rabbit Anti- Cav-1抗體說(shuō)明書(shū),新學(xué)期*

        WB=1:100-500  
        ELISA=1:500-1000  
        IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500 ,not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user..
Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃
 
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Rabbit Anti- Cav-1抗體說(shuō)明書(shū),新學(xué)期*


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