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Remel™ MUG 紙片

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Thermo Scientific Remel MUG 紙片用于采用熒光測(cè)定法對(duì)大腸桿菌進(jìn)行推斷性鑒定。

詳細(xì)介紹

Godsey et al. supported their findings in an evaluation of methods based on microbial enzyme activity profiles³. Most strains of E. coli produce an enzyme β-D-glucuronidase. In the indole test, the enzyme tryptophanase attacks tryptophan molecule on its side chain, leaving the aromatic ring in the form of indole. The indole is then detected by addition of ρ-dimethylamino-benzaldehyde (Kavocs’ Reagent) which produces a red color. β-D-glucuronidase is an enzyme that cleaves the substrate 4-methylumbelliferyl-β-D-glucuronide (MUG) and thereby produces a fluorescent product called methylumbelliferone⁴. This compound is detectable using a long wave ultraviolet light (360 nm).

Ready to use - 4-methylumbelliferyl- β-D-glucuronide-impregnated disk
Easy to store - No special storage required
Easy to interpret - With visual inspection
- In MUG Disk test, examine the disk for fluorescence using a longwave ultraviolet light (360nm) in a darkened room. Blue fluorescence is interpreted as positive test.
- In Indole test, add one drop of Kovacs‘ Indole Reagent to the disk and immediately observe for red color development.

Interpretation:

MUG Disk Test (Direct or Tube Test):

Positive test - Blue fluorescence observed under 360 nm UV light

Negative test - No fluorescence observed under 360 nm UV light

Indole Test:

Positive test - Red color development on the disk or in the tube

Negative test - No red color development on the disk or in the tube

Kilian, M. and P. Bulow. 1976. Acta Pathol. Microbiol. Scand. Sect. B. 84:245-251.
Trepta, R.W. and S.C. Edberg. 1984. J. Clin. Microbiol. 19:172-174.
Godsey, J.H., M.R. Matteo, D. Shen, G. Tolman, and J.R. Gohlke. 1981. J. Clin. Microbiol. 13:483-490.
Raj, P. 1993. Clin. Microbiol. Newsl. 15:89-93.