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當(dāng)前位置：珀金埃爾默醫(yī)學(xué)診斷產(chǎn)品（上海）有限公司>>>>[Methyl-14C] Methylated, Protein Molecular Weight Markers, 1µCi (37kBq)

[Methyl-14C] Methylated, Protein Molecular Weight Markers, 1µCi (37kBq)

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更新時(shí)間：2022-10-15 15:24:35瀏覽次數(shù)：80次

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14C-labeled markers are for use in radiometric applications. The eletrophoretic mobility of methylated molecular weight markers is identical to the unmodified individual protein.

詳細(xì)介紹

14C-labeled protein molecular weight markers for gel electrophoresis and autoradiography. Gel electophoresis is a widely employed technique for determining the molecular weight of proteins. The mobility of a protein on polyacrylamide gels containing sodium dodecylsulfate (SDS) is primarily a function of the size of the macromolecule. 0.02 µCi of each marker is sufficient for overnight detection by autogradiography.

  • Phosphorylase B: 97,400 Da
  • Albumin (BSA): 69,000 Da
  • Gammaglobulin subunit 1: 55,400 Da
  • Gammaglobulin subunit 2: 30,000 Da
  • Ovalbumin: 46,000 Da
  • Cytochrome C: 12,300 Da

Sample preparation for electrophoresis. Just prior to use:

  1. Make sure the solution is homogeneous. Some precipitation of the SDS is likely to occur upon thawing. It can be brought back to solution by gently warming the solution to 37 °C.
  2. Add 2-mercaptoethanol and SDS to your sample loading buffer (5% by volume) in a snap cap polypropylene tube and place in a boiling water bath for 3-5 minutes.

規(guī)格

濃度 0.005 mCi/mL
檢測(cè)方法 Radiometric
標(biāo)簽位置 Specifically Labeled
放射性同位素 C-14
放射性核素 14C
運(yùn)輸條件 干冰
特殊訂購(gòu)信息 This is a radioactive product - shipping address must have a license to receive radioactive materials.
產(chǎn)品尺寸 1 µCi

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