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ACQUITY UPLC HSS C18 （SB）Column

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疏水反相保留機(jī)理、硅膠顆粒

詳細(xì)介紹

與CORTECS，CSH和BEH顆粒相比，ACQUITY UPLC HSS色譜柱為極性與非極性分析物提供更理想、更平衡的保留效果。經(jīng)過(guò)工藝優(yōu)化的硅膠顆粒，其機(jī)械強(qiáng)度與穩(wěn)定性更能耐受。UPLC系統(tǒng)操作壓力。5種差異較大的鍵合相，提供更寬泛的選擇空間，除了傳統(tǒng)的疏水反相保留機(jī)理之外，還有偶極-偶極、芳香與氫鍵相互作用。


ACQUITY UPLC HSS C18 SB色譜柱是一種未封端、低覆蓋度硅膠基質(zhì)C18化學(xué)，可為受硅醇基作用影響的化合物提供其它選擇性。HSS C18 SB色譜柱硅醇活性提高，使得堿性化合物的保留更強(qiáng)(由于與殘留硅醇基的次級(jí)作用)，同時(shí)降低了對(duì)非堿性分析物的保留

(由于其低配體密度以及離子推斥性)。該保留特征的組合產(chǎn)生了一種有用的方法開(kāi)發(fā)工具，可為堿性分析物提供不同的選擇性(SB：Selectivity for Bases)，同時(shí)還可保持優(yōu)異峰形。


Development of a sensitive UPLC-ESI-MS/MS method for quantification of sofosbuvir and its metabolite, GS-331007, in human plasma: Application to a bioequivalence study


A rapid and simple LC-MS/MS method was developed and validated for the simultaneous estimation of sofosbuvir (SF) and its metabolite GS-331007 (GS) using famotidine as an internal standard (IS). The Xevo TQD LC-MS/MS was operated under the multiple-reaction monitoring mode using electrospray ionization. Extraction with ethyl acetate was used in sample preparation. The prepared samples were chromatographed on Acquity UPLC HSS C18 (50 mm × 2.1 mm, 1.8 μm) column by pumping 0.1% formic acid and acetonitrile (50:50, v/v) in an isocratic mode at a flow rate of 0.3 ml/min. Method validation was performed as per the FDA guidelines and the standard curves were found to be linear in the range of 10–2500 ng/ml for both SF and its metabolite. The intra-day and inter-day precision and accuracy results were within the acceptable limits. A very short run time of 1.2 min made it possible to analyze more than 300 human plasma samples per day. The developed assay method was successfully applied to a bioequivalence study in human volunteers.

品名

編號(hào)

ACQUITY - Xselect HSS C18 Method Transfer Kit, 100?, 1.8 - 5 µm, 2.1 - 4.6 mm X 50 - 100mm, 2/pkg

186004968

ACQUITY - Xselect HSS C18 Method Transfer Kit, 100?, 1.8 - 5 µm, 2.1 - 4.6 mm X 50 - 150mm, 2/pkg

186004961

ACQUITY - Xselect HSS C18 SB Method Transfer Kit, 100?, 1.8 - 3.5 µm, 2.1 - 4.6 mm X 50 - 100mm, 2...

186004970

ACQUITY UPLC HSS C18 Column, 100?, 1.8 µm, 1 mm X 100 mm, 1/pkg

186003530

ACQUITY UPLC HSS C18 Column, 100?, 1.8 µm, 1 mm X 150 mm, 1/pkg

186003531

ACQUITY UPLC HSS C18 Column, 100?, 1.8 µm, 3 mm X 30 mm, 3/pkg

176001817

ACQUITY UPLC HSS C18 Column, 100?, 1.8 µm, 3 mm X 50 mm, 3/pkg

176001818

ACQUITY UPLC HSS C18 Column, 100?, 1.8 µm, 3 mm X 100 mm, 3/pkg

176001819

ACQUITY UPLC HSS C18 Column, 100?, 1.8 µm, 1 mm X 50 mm, 3/pkg

176001121

ACQUITY UPLC HSS C18 Column, 100?, 1.8 µm, 2.1 mm X 30 mm, 3/pkg

176001398

ACQUITY UPLC HSS C18 Column, 100?, 1.8 µm, 2.1 mm X 150 mm, 3/pkg

176001126

ACQUITY UPLC HSS C18 Column, 100?, 1.8 µm, 1 mm X 100 mm, 3/pkg

176001122

ACQUITY UPLC HSS C18 Column, 100?, 1.8 µm, 1 mm X 150 mm, 3/pkg

176001123

ACQUITY UPLC HSS C18 Method Validation Kit, 100?, 1.8 µm, 2.1 mm X 150 mm, 3/pkg

186006026

ACQUITY UPLC HSS C18 Column, 100?, 1.8 µm, 2.1 mm X 50 mm, 3/pkg

176001124

ACQUITY UPLC HSS C18 Column, 100?, 1.8 µm, 2.1 mm X 100 mm, 3/pkg

176001125

ACQUITY UPLC HSS C18 Column, 100?, 1.8 µm, 2.1 mm X 150 mm, 1/pkg

186003534

ACQUITY UPLC HSS C18 Column, 100?, 1.8 µm, 2.1 mm X 100 mm, 1/pkg

186003533

ACQUITY UPLC HSS C18 Column, 100?, 1.8 µm, 2.1 mm X 30 mm, 1/pkg

186003987

ACQUITY UPLC HSS C18 Column, 100?, 1.8 µm, 2.1 mm X 50 mm, 1/pkg

186003532

ACQUITY UPLC HSS C18 Column, 100?, 1.8 µm, 3 mm X 30 mm, 1/pkg

186004682

ACQUITY UPLC HSS C18 Column, 100?, 1.8 µm, 3 mm X 50 mm, 1/pkg

186004683

ACQUITY UPLC HSS C18 Column, 100?, 1.8 µm, 3 mm X 100 mm, 1/pkg

186004684

ACQUITY UPLC HSS C18 Column, 100?, 1.8 µm, 3 mm X 150 mm, 1/pkg

186004685

ACQUITY UPLC HSS C18 Column, 100?, 1.8 µm, 3 mm X 75 mm, 1/pkg

186005618

ACQUITY UPLC HSS C18 Column, 100?, 1.8 µm, 2.1 mm X 75 mm, 1/pkg

186005615

ACQUITY UPLC HSS C18 Column, 100?, 1.8 µm, 3 mm X 150 mm, 3/pkg

176001820

ACQUITY UPLC HSS C18 Column, 100?, 1.8 µm, 1 mm X 50 mm, 1/pkg

186003529

ACQUITY UPLC HSS C18 VanGuard Pre-column, 100?, 1.8 µm, 2.1 mm X 5 mm, 3/pkg

186003981

ACQUITY UPLC HSS C18 SB Column, 100?, 1.8 µm, 3 mm X 150 mm, 1/pkg

186004689

ACQUITY UPLC HSS C18 SB Column, 100?, 1.8 µm, 2.1 mm X 30 mm, 3/pkg

176001559

ACQUITY UPLC HSS C18 Method Validation Kit, 100?, 1.8 µm, 2.1 mm X 50 mm, 3/pkg

186004057

ACQUITY UPLC HSS C18 SB Column, 100?, 1.8 µm, 1 mm X 50 mm, 3/pkg

176001556

ACQUITY UPLC HSS C18 SB Column, 100?, 1.8 µm, 1 mm X 100 mm, 3/pkg

176001557

ACQUITY UPLC HSS C18 SB Column, 100?, 1.8 µm, 1 mm X 150 mm, 3/pkg

176001558

ACQUITY UPLC HSS C18 SB Column, 100?, 1.8 µm, 3 mm X 100 mm, 1/pkg

186004826

ACQUITY UPLC HSS C18 SB Column, 100?, 1.8 µm, 2.1 mm X 100 mm, 1/pkg

186004119

ACQUITY UPLC HSS C18 SB Column, 100?, 1.8 µm, 2.1 mm X 30 mm, 1/pkg

186004117

ACQUITY UPLC HSS C18 SB Column, 100?, 1.8 µm, 2.1 mm X 50 mm, 1/pkg

186004118

ACQUITY UPLC HSS C18 SB Column, 100?, 1.8 µm, 1 mm X 100 mm, 1/pkg

186004115

ACQUITY UPLC HSS C18 SB Column, 100?, 1.8 µm, 1 mm X 150 mm, 1/pkg

186004116

ACQUITY UPLC HSS C18 SB Column, 100?, 1.8 µm, 3 mm X 30 mm, 1/pkg

186004686

ACQUITY UPLC HSS C18 SB Column, 100?, 1.8 µm, 3 mm X 100 mm, 3/pkg

176001823

ACQUITY UPLC HSS C18 SB Column, 100?, 1.8 µm, 2.1 mm X 75 mm, 1/pkg

186005616

ACQUITY UPLC HSS C18 SB Column, 100?, 1.8 µm, 3 mm X 150 mm, 3/pkg

176001824

ACQUITY UPLC HSS C18 SB Column, 100?, 1.8 µm, 2.1 mm X 150 mm, 1/pkg

186004120

ACQUITY UPLC HSS C18 SB Column, 100?, 1.8 µm, 3 mm X 30 mm, 3/pkg

176001821

ACQUITY UPLC HSS C18 SB Column, 100?, 1.8 µm, 2.1 mm X 150 mm, 3/pkg

176001562

ACQUITY UPLC HSS C18 SB Column, 100?, 1.8 µm, 2.1 mm X 100 mm, 3/pkg

176001561

ACQUITY UPLC HSS C18 SB Column, 100?, 1.8 µm, 2.1 mm X 50 mm, 3/pkg

176001560

ACQUITY UPLC HSS C18 Method Validation Kit, 100?, 1.8 µm, 3 mm X 100 mm, 3/pkg

186004704

ACQUITY UPLC HSS C18 Method Validation Kit, 100?, 1.8 µm, 3 mm X 50 mm, 3/pkg

186004703

ACQUITY UPLC HSS C18 SB VanGuard Pre-column, 100?, 1.8 µm, 2.1 mm X 5 mm, 3/pkg

186004136

ACQUITY UPLC HSS C18 SB Method Validation Kit, 100?, 1.8 µm, 2.1 mm X 150 mm, 3/pkg

186006027

ACQUITY UPLC HSS C18 SB Method Validation Kit, 100?, 1.8 µm, 2.1 mm X 50 mm, 3/pkg

186004137

ACQUITY UPLC HSS C18 SB Method Validation Kit, 100?, 1.8 µm, 2.1 mm X 100 mm, 3/pkg

186004138

ACQUITY UPLC HSS C18 SB Column, 100?, 1.8 µm, 3 mm X 50 mm, 3/pkg

176001822

ACQUITY UPLC HSS C18 SB Column, 100?, 1.8 µm, 3 mm X 75 mm, 1/pkg

186005619

ACQUITY UPLC HSS C18 SB Method Validation Kit, 100?, 1.8 µm, 3 mm X 50 mm, 3/pkg

186004705

ACQUITY UPLC HSS C18 SB Method Validation Kit, 100?, 1.8 µm, 3 mm X 100 mm, 3/pkg

186004709


關(guān)鍵詞：色譜柱
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