A549細(xì)胞株

A549細(xì)胞株 人非小細(xì)胞肺癌細(xì)胞系是1972年由GiardDJ通過肺癌組織移植培養(yǎng)建系的，源自一位58歲的白人男性。A549能通過胞苷二磷脂酰途徑合成富含不飽和脂肪酸的；角蛋白陽性。

細(xì)胞名稱：人非小細(xì)胞肺癌細(xì)胞

細(xì)胞別稱： A 549; A549; NCI-A549; A549/ATCC; A549 ATCC; A549-ATCC; hA549

細(xì)胞簡稱：A549細(xì)胞株

產(chǎn)品貨號：TCH-C116

種屬來源：人

組織來源：肺

特征：肺癌

細(xì)胞形態(tài)：上皮細(xì)胞樣

生長特性：貼壁生長

培養(yǎng)體系：D/F12+10%FBS+1%P/S

A549細(xì)胞培養(yǎng)基貨號：TCH-G116

傳代比例：1:3-1:6，每2-3天換液一次

傳代周期：48-72 h

培養(yǎng)條件：氣相：95%空氣+5%CO2，溫度：37℃

凍存條件：60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO，液氮儲存

質(zhì)量檢測：細(xì)菌、真菌、支原體檢測均為陰性

實(shí)驗(yàn)室儀器及培養(yǎng)環(huán)境：CO2培養(yǎng)箱 細(xì)胞培養(yǎng)瓶 細(xì)胞培養(yǎng)皿

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