u2os細(xì)胞

細(xì)胞名稱： 人骨肉瘤細(xì)胞（STR鑒定正確）

細(xì)胞別稱： U2OS,U-2 OS

細(xì)胞簡(jiǎn)稱：u2os細(xì)胞

貨號(hào)：TCH-C365

規(guī)格：1×10?cells/T25 培養(yǎng)瓶

價(jià)格：1000元 (干冰運(yùn)輸需另加200元干冰費(fèi))

種屬來(lái)源：人

組織來(lái)源：骨

疾病特征：骨肉瘤

細(xì)胞形態(tài)：上皮細(xì)胞樣

生長(zhǎng)特性：貼壁生長(zhǎng)

培養(yǎng)體系：McCoy's 5A+10%FBS+1%P/S

配套培養(yǎng)基貨號(hào)：TCH-G365

傳代比例：1:3-1:6，每2-3天換液一次

傳代周期：48-72 h

u2os細(xì)胞 培養(yǎng)條件：氣相：95%空氣+5%CO2，溫度：37℃

凍存條件：60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO，液氮儲(chǔ)存

質(zhì)量檢測(cè)：細(xì)菌、真菌、支原體檢測(cè)均為陰性

u2os細(xì)胞規(guī)格

   u2os細(xì)胞系(原名2T)是由PontenJ和SakselaE在1964年從一名15歲女孩的脛骨中等分化的肉瘤中分離建立的。與WI-38共培養(yǎng)或用抗猴空泡病毒SV40、呼吸道合胞病毒RSV或腺病毒的補(bǔ)體結(jié)合試驗(yàn)（CF法）未檢測(cè)到病毒。該細(xì)胞表達(dá)胰島素樣生長(zhǎng)因子Ⅰ、Ⅱ（IGF-Ⅰ、IGF-Ⅱ）受體和骨肉瘤衍生生長(zhǎng)因子（ODGF）。

Gao Y, Zhu Y, Wang H, et al. Lipid-mediated phase separation of AGO proteins on the ER controls nascent-peptide ubiquitination[J]. Molecular Cell, 2022, 82(7): 1313-1328. e8.

Heldin CH, et al. A human osteosarcoma cell line secretes a growth factor structurally related to a homodimer of PDGF A-chains. Nature 319: 511-514, 1986. PubMed: 3456080

Ponten J, Saksela E. Two established in vitro cell lines from human mesenchymal tumours. Int. J. Cancer 2: 434-447, 1967. PubMed: 6081590

Raile K, et al. Human osteosarcoma (U-2 OS) cells express both insulin-like growth factor-I (IGF-I) receptors and insulin-like growth factor-II/mannose-6- phosphate (IGF-II/M6P) receptors and synthesize IGF-II: autocrine growth stimulation by IGF-II via the IGF-I receptor. J. Cell. Physiol. 159 : 531-541 , 1994. PubMed: 8188767

Landers JE, et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed: 9270029

Moradpour D, et al. Characterization of cell lines allowing titghtly regulated expression of heapatitis C virus core protein. Virology 222: 51-63, 1996. PubMed: 8806487

Ponten J, Saksela E. Two established in vitro cell lines from human mesenchymal tumours. Int. J. Cancer 2: 434-447, 1967. PubMed: 6081590"