MHCC97H-人肝癌細(xì)胞

細(xì)胞名稱(chēng)：MHCC97H-人肝癌細(xì)胞 （STR鑒定正確）

細(xì)胞別名：  MHCC 97-H; MHCC-97H; MHCC97H

細(xì)胞簡(jiǎn)稱(chēng)：MHCC97H

產(chǎn)品貨號(hào)：TCH-C258

產(chǎn)品規(guī)格： 1×10?cells/T25 培養(yǎng)瓶

種屬來(lái)源：人

組織來(lái)源：肝

特征：高轉(zhuǎn)移潛能肝癌細(xì)胞

細(xì)胞形態(tài)：上皮細(xì)胞樣

生長(zhǎng)特性：貼壁生長(zhǎng)

培養(yǎng)體系：DMEM-H+10%FBS+1%Glutamax+1% Sodium Pyruvate+1%P/S

MHCC97H-人肝癌細(xì)胞 配套培養(yǎng)基貨號(hào)：TCH-G258

傳代比例：1:3-1:6，每2-3天換液一次

傳代周期：48-72 h

培養(yǎng)條件：氣相：95%空氣+5%CO2，溫度：37℃

凍存條件：60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO，液氮儲(chǔ)存

質(zhì)量檢測(cè)：細(xì)菌、真菌、支原體檢測(cè)均為陰性

 推薦使用T25瓶，復(fù)蘇發(fā)貨1-2周

MHCC97H-人肝癌細(xì)胞

參考文獻(xiàn)：

1. Dang H., Steinway S.N., Ding W., Rountree C.B.

Induction of tumor initiation is dependent on CD44s in c-Met(+) hepatocellular carcinoma.

BMC Cancer 15:161-161(2015)

2. Tian M.-M., Cheng H., Wang Z.-Q., Su N., Liu Z.-X., Sun C.-Q., Zhen B., Hong X.-C., Xue Y., Xu P.

Phosphoproteomic analysis of the highly-metastatic hepatocellular carcinoma cell line, MHCC97-H.

Int. J. Mol. Sci. 16:4209-4225(2015)

3. Wang K., Lim H.Y., Shi S., Lee J., Deng S., Xie T., Zhu Z., Wang Y., Pocalyko D., Yang W.J., Rejto P.A., Mao M., Park C.-K., Xu J.

Genomic landscape of copy number aberrations enables the identification of oncogenic drivers in hepatocellular carcinoma.

Hepatology 58:706-717(2013)

4. Li Y., Tian B., Yang J., Zhao L., Wu X., Ye S.-L., Liu Y.-K., Tang Z.-Y.

Stepwise metastatic human hepatocellular carcinoma cell model system with multiple metastatic potentials established through consecutive in vivo selection and studies on metastatic characteristics.

J. Cancer Res. Clin. Oncol. 130:460-468(2004)

5. Ding S.-J., Li Y., Shao X.-X., Zhou H., Zeng R., Tang Z.-Y., Xia Q.-C.

Proteome analysis of hepatocellular carcinoma cell strains, MHCC97-H and MHCC97-L, with different metastasis potentials.

Proteomics 4:982-994(2004)