LysoBrite 溶酶體近紅外熒光探針，用于標(biāo)記活細(xì)胞的溶酶體。專有的溶解性染料可能通過溶酶體pH梯度選擇性地積聚在溶酶體中。溶質(zhì)指示劑是疏水性化合物，易于滲透完整的活細(xì)胞，并在進(jìn)入細(xì)胞后被捕獲在溶酶體中。進(jìn)入溶酶體后，LysoBrite 試劑的熒光明顯增強(qiáng)。可以使用熒光成像，高含量成像，酶標(biāo)儀熒光測(cè)定法或流式細(xì)胞術(shù)檢測(cè)熒光。 LysoBrite 橙色，紅色，深紅色和NIR試劑具有很高的光穩(wěn)定性和優(yōu)異的細(xì)胞保留性，可用于各種研究，包括細(xì)胞粘附，趨化性，多藥耐藥性，細(xì)胞活力，細(xì)胞凋亡和細(xì)胞毒性。它們適用于增殖和非增殖細(xì)胞，可用于懸浮細(xì)胞和貼壁細(xì)胞。溶酶體是細(xì)胞器，其含有酸水解酶以分解廢物和細(xì)胞碎片。溶酶體消化多余的或破舊的細(xì)胞器，食物顆粒和吞噬的病毒或細(xì)菌。溶酶體周圍的膜允許消化酶在pH4.5下工作。與微堿性胞質(zhì)溶膠（pH 7.2）相比，溶酶體的內(nèi)部是酸性的（pH 4.5-4.8）。溶酶體通過質(zhì)子泵和氯離子通道從細(xì)胞質(zhì)中泵送質(zhì)子穿過膜來維持這種pH差異。

2.百螢 LysoBrite溶酶體近紅外熒光探針參數(shù)

Ex(nm) 636

Em(nm) 651

分子量 730.89

溶劑 DMSO

存儲(chǔ)條件在-15℃以下保存，避光防潮

3.百螢 LysoBrite溶酶體近紅外熒光探針參考文獻(xiàn)

Anti-tumour activity of low molecular weight heparin doxorubicin nanoparticles for histone H1 high-expressive prostate cancer PC-3M cells
Authors: Shuang Zhang, Zhan-Tao Li, Man Liu, Jing-Ru Wang, Mei-Qi Xu, Zhuo-Yue Li, Xiao-Chuan Duan, Yan-Li Hao, Xiu-Chai Zheng, Hui Li
Journal: Journal of Controlled Release (2018)

Autophagy proteins are not universally required for phagosome maturation
Authors: Marija Cemma, Sergio Grinstein, John H Brumell
Journal: Autophagy (2016): 1440--1446

Differential detection of tumor cells using a combination of cell rolling, m*lent binding, and multiple antibodies
Authors: Ja Hye Myung, Khyati A Gajjar, Jihua Chen, Robert E Molokie, Seungpyo Hong
Journal: Analytical chemistry (2014): 6088--6094

Versatile fabrication of nanoscale sol--gel bioactive glass particles for efficient bone tissue regeneration
Authors: Bo Lei, Xiaofeng Chen, Xue Han, Jiaan Zhou
Journal: Journal of Materials Chemistry (2012): 16906--16913

Advanced glycation end-products increase IL-6 and ICAM-1 expression via RAGE, MAPK and NF-$\kappa$B pathways in human gingival fibroblasts
Authors: K Nonaka, Y Kajiura, M Bando, E Sakamoto, Y Inagaki, JH Lew, K Naruishi, T Ikuta, K Yoshida, T Kobayashi
Journal: Journal of Periodontal Research