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上海研盟生物科技有限公司Anti-REA抗體*，主要應(yīng)用于WB、IHC、IF、ELISA、流式細(xì)胞術(shù)等實(shí)驗(yàn)中。說明書隨貨發(fā)送，您也可以直接我司在線客服索取。客服

英文名稱：Anti-REA antibody

中文名稱：B淋巴細(xì)胞受體相關(guān)蛋白抗體

B淋巴細(xì)胞受體相關(guān)蛋白抗體保存條件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

★研盟生物★B淋巴細(xì)胞受體相關(guān)蛋白抗體,Anti-REA抗體

免疫組化操作步驟

（一）、儀器設(shè)備

1、 18cm不銹鋼高壓鍋或電爐或用微波爐

2、 水浴鍋

（二）、試 劑

1、 PBS緩沖液（pH7.2―7.4）

2、 0.01mol/L檸檬酸鈉緩沖液（CB,pH6.0,1000ml）

3、 3%甲醇―H2O2溶液：用30% H2O2 和80%甲醇溶液配制

4、 風(fēng)裱劑：

a. 甘油和0.5mmol/L碳酸鹽緩沖液（pH9.0–9.5）等量混合

b. 油和TBS（PBS）配制

5、 TBS/PBS pH9.0–9.5，適用于熒光纖維鏡標(biāo)本；pH7.0-7.4適合光學(xué)纖維標(biāo)本

（三）、操作流程

1、 脫蠟和水化：脫蠟前應(yīng)將組織芯片在室溫中放置60分鐘或60℃恒溫箱中烘烤20分鐘

a. 組織芯片置于二甲苯中浸泡10分鐘，更換二甲苯后在浸泡10分鐘

b. 無水乙醇中浸泡五分鐘

c. 95%乙醇中浸泡五分鐘

d. 75%乙醇中浸泡五分鐘

2、 抗原修復(fù)：用于福爾馬林固定的石蠟包埋組織芯片

B淋巴細(xì)胞受體相關(guān)蛋白抗體,Anti-REA抗體溶解方法:

方法1：我們的抗體（凍干粉）已經(jīng)包含了0.01M PBS、1% BSA和0.1% 防腐劑（疊氮鈉或*），您只需要加入滅菌的雙蒸水就行了?？贵w溶解后，置于-20℃保存，分裝后使用，避免反復(fù)凍融，可保證至少1年有效；

方法2：也可以只加入一半體積的雙蒸水，再用一半體積的甘油補(bǔ)足，置于-20℃保存，這樣抗體不會(huì)凍，有利于抗體的穩(wěn)定。

后續(xù)試驗(yàn)時(shí)，再使用對(duì)應(yīng)的緩沖液稀釋成工作液，即用即配。

我們的抗體（凍干粉），在常溫放置下，至少一個(gè)月有效；若在-20℃下保存（*），至少三年有效。

Antibodies are prepared with 0.01M PBS, pH 7.4 with 1% BSA and 0.1% Sodium Azide, prior to lyophilization. Currently, we offer both lyophilized and reconstituted antibodies, both resulting in final concentration of 1mg/mL. We recommend two ways to reconstitute antibodies in lyophilized form:

1) Reconstituting by directly adding 100uL of sterile, distilled water.

2) Alternatively, if glycerol does not affect the experiment, add 50uL sterile distilled water, followed by adding 50uL of glycerol.

簡單介紹：

產(chǎn)品別名：B cell receptor associated protein BAP37; B-cell associated protein; B-cell receptor-associated protein BAP37; Bap 37; BAP; Bap37; BCAP 37; BCAP37; D prohibitin; D-prohibitin; MGC117268; p22; PHB 2; PHB2; PHB2_HUMAN; PNAS 141; PNAS141; Prohibitin 2; Prohibitin-2; Prohibitin2; Repressor of Estrogen Receptor Activity.      

抗體來源：Rabbit or Mouse

保質(zhì)期:1年

克隆類型：Polyclonal or monoclonal

性    狀：Lyophilized or Liquid

濃    度：1mg/1ml

背景介紹：The prohibitins, called PHB1 and PHB2, are highly conserved proteins that are present in nuclear and mitochondrial compartments in eukaryotic cells and exert multiple cellular functions (1). PHB2 (REA) was originally identified as an estrogen receptor (ER) specific coregulator. It directly interacts with hormone-bound ER and represses its transcriptional activity by competitive inhibition of SRC-1 coactivation of ER (2,3). Together with COUP-TFs, PHB2 also interacts with histone deacetylases HDAC1 and HDAC5, and mediates transcriptional regulation by ER through coupling the deacetylase to the transcription activation complex (4). Another important function of PHB2 is stablization of mitochondrial proteins. PHB1/PHB2 hetrodimers form large ring complexes on the mitochondrial membrane (5), acting as chaperones to stablize important mitochodrial proteins, such as OPA1 and Hax1 to support mitochondrial morphogenesis and protection against apoptosis (6-8).

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