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聯(lián)系我時(shí),請(qǐng)告知來自 環(huán)保在線“Anti-TLR4抗體,Toll樣受體4抗體"2A抗體規(guī)格0.1ml、0.2ml、1ml,抗體產(chǎn)品有效期一年,質(zhì)保三個(gè)月,質(zhì)保期內(nèi)出現(xiàn)任何質(zhì)量問題或是出不了實(shí)驗(yàn)結(jié)果均可免費(fèi)退換:
上海研盟生物科技有限公司Anti-TLR4抗體,Toll樣受體4抗體*,主要應(yīng)用于WB、IHC、IF、ELISA、流式細(xì)胞術(shù)等實(shí)驗(yàn)中。說明書隨貨發(fā)送,您也可以直接我司在線客服索取??头?/p>
簡(jiǎn)單介紹:
中文名稱:Toll樣受體4抗體
英文名稱:Anti-TLR4
產(chǎn)品編號(hào):byk-1021R
產(chǎn)品別名:TLR4; TLR-4; TLR 4; ARMD10; CD 284; CD284; CD284 antigen; Homolog of Drosophila toll; hTol; Toll (Drosophila) homolog; TOLL; Toll Endotoxin Hyporesponsiveness; Toll like receptor 4; Toll like receptor 4 precursor; TLR4_HUMAN.
產(chǎn)品規(guī)格:0.1ml、0.2ml、1ml
產(chǎn)品用途:科研實(shí)驗(yàn)
產(chǎn)品價(jià)格:請(qǐng)報(bào)價(jià)
說明書:請(qǐng)?zhí)砑涌头苯铀魅?/p>
抗體來源:該指標(biāo)有兩種產(chǎn)品,一種是兔來源抗體,一種是鼠來源抗體
克隆類型:兔來源為多克隆抗體,鼠來源單克隆抗體
交叉反應(yīng):請(qǐng)索取說明書查看
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
亞 型:IgG
純化方法: affinity purified by Protein A
儲(chǔ) 存 液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
保存條件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-TLR4抗體,Toll樣受體4抗體產(chǎn)品介紹:The Toll-like receptor 4 by activating natural immunity, specific immune response involved in the start-up, Toll-like receptor 4 as an important signal transduction transmembrane receptor involved in the toxin-induced inflammation in the pathological process, its mechanisms of control On a growing concern. all regions were either double-stranded or sequenced with an alternatechemistry or covered by high quality data(i.e., phred quality >=30); an attempt was made to resolve all sequencing problems, such as compressions and repeats; all regions were covered by at least one subclone; and the assembly was confirmed by restriction digest, except on the rare occasion of the clone being a YAC. The following abbreviations are used to associate primary accession numbers given in the feature table with their source databases: Em:, EMBL; Sw:, SWISSPROT; Tr:, TREMBL; Wp:, WORMPEP; Information on the WORMPEP database can be found at. TLR-4 plays an important role in microvascular leakage and leukocyte adhesion under the inflammatory condition associated with nonseptic thermal injury.
免疫印跡法是一種通過凝膠電泳分離蛋白質(zhì)并通過電轉(zhuǎn)移把蛋白質(zhì)轉(zhuǎn)移到吸附膜上。一經(jīng)蛋白印跡后,即可通過特異性的標(biāo)記抗體檢測(cè)到相應(yīng)蛋白質(zhì)。
十二烷基硫酸鈉-聚丙烯酰胺凝膠電泳(SDS-PAGE)可用于檢測(cè)已被SDS化學(xué)變性的蛋白。然而,某些特定的抗體不會(huì)在一個(gè)變性的蛋白質(zhì)分子上識(shí)別其抗原表位,這時(shí)需要進(jìn)行不含SDS的聚丙烯酰胺凝膠電泳。
蛋白印跡轉(zhuǎn)膜可以通過濕法或半干法進(jìn)行。在前者,凝膠夾在印跡膜和各種過濾裝置中間,然后把整個(gè)裝置埋進(jìn)一個(gè)裝滿Tris轉(zhuǎn)移緩沖的電極槽中。在后者,夾在中間的凝膠周圍只有少量的緩沖液,然后封閉在電極板之間。雖然半干法轉(zhuǎn)膜較快,但需要很好地?cái)M合各部分的夾層配件,而濕法轉(zhuǎn)膜很少會(huì)出現(xiàn)問題,可以盡量保持重復(fù)實(shí)驗(yàn)結(jié)果的*性。此外,如果半干法轉(zhuǎn)膜時(shí)間過長(zhǎng),較小分子量的蛋白質(zhì)可能會(huì)從膜上轉(zhuǎn)過。
轉(zhuǎn)膜后,需對(duì)膜進(jìn)行封閉,以減少非特異性蛋白結(jié)合,然后進(jìn)行用一抗孵育膜,以研究感興趣的目標(biāo)蛋白。單克隆抗體通常具有更高的特異性;然而許多單克隆抗體產(chǎn)生于某個(gè)特定肽段而不是一個(gè)天然蛋白質(zhì),因此可能無法檢測(cè)到PAGE所分離的天然蛋白。也可使用多克隆抗體,但易形成較高的背景值。因一抗通常無標(biāo)記,所以需要一個(gè)可以結(jié)合在一抗Fc段的標(biāo)記的二抗,以用于zui后的檢測(cè)。通常會(huì)用酶來標(biāo)記二抗。酶標(biāo)記后的印跡可通過化學(xué)發(fā)光酶底物及放射自顯影技術(shù)成像。被抗體檢測(cè)和標(biāo)記到的蛋白質(zhì)會(huì)出現(xiàn)在圖像的暗區(qū)。
相關(guān)產(chǎn)品:
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Snake poison Protein 抗蛇毒蛋白抗體(中華眼鏡蛇)
SNAP-25 (synaptosomal-associated protein 25) 突觸相關(guān)蛋白25抗體
Snail 抗Snail蛋白抗體
Socs 1 (suppressor of cytokine signaling 1) 細(xì)胞因子信號(hào)傳導(dǎo)抑制蛋白1抗體
Socs 2(suppressor of cytokine signaling 2) 細(xì)胞因子信號(hào)傳導(dǎo)抑制蛋白2抗體
Socs 3 (suppressor of cytokine signaling 3) 細(xì)胞因子信號(hào)傳導(dǎo)抑制蛋白3抗體
SOD1 (superoxide-dimutase-1) 超氧化物歧化酶1抗體
SOD2 (superoxide-dimutase-2) 超氧化物歧化酶2抗體
Solute carrier family 1 溶質(zhì)攜帶物家族-1抗體
Somatostatin/GRIH *抗體
Sox2 胚胎干細(xì)胞關(guān)鍵蛋白抗體
SP (Substance P) P物質(zhì)抗體
TSFP1/SP1(transcription factor Sp1) 轉(zhuǎn)錄生長(zhǎng)因子SP1抗體
TPA/PLAT 組織型纖溶酶原激活劑抗體
SP-A (pulmonary surfactant-associated glycoprotein A) 肺表面活性蛋白A抗體
SPA-1/SIPA-1(signal-induced proliferation-associated protein-1) 抗信號(hào)感應(yīng)增殖相關(guān)蛋白 1抗體
SPAG5/map126(sperm associated gen 5) 抗精zi相關(guān)抗原5抗體
SPARC (secreted protein acidic and rich in cysteine) 富含半*的酸性分泌蛋白抗體
SP-B (pulmonary surfactant-associated glycoprotein B) 肺表面活性蛋白B抗體
SP-D (pulmonary surfactant-associated glycoprotein D) 肺表面活性蛋白D抗體
Spectrin-α (Spna 2 protein / Spectrin alpha) 血影蛋白/收縮蛋白抗體
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