Pierce Copper Coated Plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carbox......
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Thermo Scientific Pierce Copper Coated High Binding Capacity (HBC) Plates use an exclusive coating process to increase the amount of histidine-tagged protein that will bind to the plate surface.
Features of Copper Coated High Binding Capacity Plates include: • Quantitate previously undetectable histidine-tagged proteins • Wider dynamic range with four-fold greater capacity than regular nickel chelate-coated plate • Copper chelate provides greater binding capacity than nickel • The detection limit is 0.1 pg of polyhistidine tagged fusion protein per well • Pre-blocked for the ultimate convenience
Note: Copper coated plates are for use with purified His-tagged proteins. Typical cell lysates contain non-his-tagged proteins that may bind to copper, which can reduce binding capacity and signal-to-noise ratios. For cell lysates, use Thermo Scientific Pierce Nickel Coated Plates.