HE021
人凝集蛋白家族11(COLEC11)ELISA試劑盒
檢測(cè)范圍：
20pg/ml-1500pg/ml
實(shí)驗(yàn)原理：
本試劑盒用于測(cè)定人血清，細(xì)胞上清及相關(guān)液體樣本中凝集蛋白家族 11（COLEC11）的含量。本試劑盒應(yīng)用雙抗體夾心法測(cè)定標(biāo)本中人凝集蛋白家族 11（COLEC11）水平。用純化的
人凝集蛋白家族 11（COLEC11）抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依
次加入凝集蛋白家族 11，再與 HRP 標(biāo)記的羊抗人抗體結(jié)合，形成抗體-抗原-酶標(biāo)抗體復(fù)合
物，經(jīng)過*洗滌后加底物 TMB 顯色。TMB 在 HRP 酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作
用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的凝集蛋白家族 11（COLEC11）呈正相關(guān)。
用酶標(biāo)儀在 450nm 波長(zhǎng)下測(cè)定吸光度（OD 值），通過標(biāo)準(zhǔn)曲線計(jì)算樣品中人凝集蛋白家族
11（COLEC11）含量。
人凝集蛋白家族11(COLEC11)ELISA試劑盒組成：
試劑盒組成 48 孔配置 96 孔配置 保存
說明書 1 份 1 份
封板膜 2 片（48） 2 片（96）
密封袋 1 個(gè) 1 個(gè)
酶標(biāo)包被板 1×48 1×96 2-8℃保存
標(biāo)準(zhǔn)品：1800pg/ml 0.5ml×1 瓶 0.5ml×1 瓶 2-8℃保存
標(biāo)準(zhǔn)品稀釋液 1.5ml×1 瓶 1.5ml×1 瓶 2-8℃保存
酶標(biāo)試劑 3 ml×1 瓶 6 ml×1 瓶 2-8℃保存
樣品稀釋液 3 ml×1 瓶 6 ml×1 瓶 2-8℃保存
顯色劑 A 液 3 ml×1 瓶 6 ml×1 瓶 2-8℃保存
顯色劑 B 液 3 ml×1 瓶 6 ml×1 瓶 2-8℃保存
終止液 3ml×1 瓶 6ml×1 瓶 2-8℃保存
濃縮洗滌液 （20ml×20 倍）×1 瓶 （20ml×30 倍）×1 瓶 2-8℃保存
Generic Name：Human Collectin sub family member 11(COLEC11) ELISAKit.
Assay range:
20pg/ml-1500pg/ml
Purpose
This kit allows for the determination of COLEC11 concentrations in Human serum, cell
culture supernatant, and other biological fluids.
Principle of the assay
T he kit assay Human COLEC11 level in the sample，use Purified Human COLEC11
antibody to coat microtiter plate wells, make solid-phase antibody, then add COLEC11 to wells,Combined antibody which With HRP labeled goat anti-Human become antibody - antigen -enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of COLEC11 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
5 Materials provided with the kit
Materials provided with the kit:
48determinations 96 determinations Storage
User manual 1 1
Closure plate membrane 2 2
Sealed bags 1 1
Microelisa stripplate 1 1 2-8℃
Standard ：1800pg/ml 0.5ml×1 bottle 0.5ml×1 bottle 2-8℃
Standard diluent 1.5ml×1 bottle 1.5ml×1 bottle 2-8℃
HRP-Conjugate reagent 3ml×1 bottle 6ml×1 bottle 2-8℃
Sample diluent 3ml×1 bottle 6ml×1 bottle 2-8℃
Chromogen SolutionA 3ml×1 bottle 6ml×1 bottle 2-8℃
Chromogen Solution B 3ml×1 bottle 6ml×1 bottle 2-8℃
Stop Solution 3ml×1 bottle 6ml×1 bottle 2-8℃
wash solution
（20ml×20 fold ）
×1 bottle
（20ml×30 fold ）
×1 bottle
2-8℃
人凝集蛋白家族11(COLEC11)ELISA試劑盒供應(yīng)商：上海華壹生物科技有限公司
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