大鼠丙二醛(MDA)ELISA試劑盒
Rat malondialchehyche(MDA)ELISA Kit
實(shí)驗(yàn)原理：本試劑盒應(yīng)用雙抗體夾心法測(cè)定標(biāo)本中大鼠丙二醛（MDA）水平。用純化的抗-丙二醛（MDA）抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入大鼠丙二醛（MDA），再與HRP標(biāo)記的MDA抗體結(jié)合，形成抗體-抗原-酶標(biāo)抗體復(fù)合物，經(jīng)過*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的大鼠丙二醛（MDA）呈正相關(guān)。用酶標(biāo)儀在450nm波長下測(cè)定吸光度（OD值），通過標(biāo)準(zhǔn)曲線計(jì)算樣品中大鼠丙二醛（MDA）濃度。
Principle of the assay
The kit assay Rat MDA level in the sample，use Purified Rat MDA to coat microtiter plate wells, make solid-phase antibody, then add MDA to wells, Combined MDA antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MDA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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