人皮質(zhì)酮(CORT)ELISA試劑盒
實(shí)驗(yàn)原理：
本試劑盒用于測定人血清，細(xì)胞上清及相關(guān)液體樣本中皮質(zhì)酮 (CORT)的含量。應(yīng)用雙抗體夾心法測定標(biāo)本中人皮質(zhì)酮 (CORT)水平。用純化的人皮質(zhì)酮 (CORT)抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入皮質(zhì)酮 (CORT)，再與HRP標(biāo)記的皮質(zhì)酮 (CORT)抗體結(jié)合，形成抗體-抗原-酶標(biāo)抗體復(fù)合物，經(jīng)過*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的人皮質(zhì)酮 (CORT)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（OD值），通過標(biāo)準(zhǔn)曲線計算樣品中人皮質(zhì)酮 (CORT)濃度。
Human Corticosterone (CORT) ELISA Kit
Principle of the assay
This kit allows for the determination of CORT concentrations in Human serum, cell culture supernatant, and other biological fluids.Assay Human CORT level in the sample，use Purified Human CORT to coat microtiter plate wells, make solid-phase antibody, then add CORT to wells, Combined antibody which With HRP labeled goat anti-Mouse become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CORT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
上海華壹生物科技有限公司
Shanghai HuaYi Bio-technology Co.,Ltd
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