Anti- TFR /FITC

Catalog Number : RSF-1782R FITC

Quantity size : 100ug （2mg/ml,Lyophilized. Buffer = 0.01M PBS, pH 7.4 with 10mg/ml BSA and 0.1% Sodium azide. ）

Relevance: Cellular uptake of iron occurs via receptor mediated endocytosis of ligand occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system （By similarity）. Useful in studies of dividing haematopoietic and tumour cell populations, and metabolic activity. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C terminal binding site. The antigen is present on most dividing cells, including normally cycling in vivo hematopoietic progenitor cells, mitogenically stimulated cells in vitro, some primary tumor cells and most proliferating cells in vitro.

Specificity : · anti- TFR is a Mouse monoclonal antibody, /FITC

           · specific for TFR of  human 

           · Protein G affinity chromatography purification, purity :>95%

· Isotype: IgG 

· mol wt:85kDa

Application : · FCM: 1:20-100

.IF=1:50-200

   .ICC=1:50-200

            · Immunohistochemistry : 1:20-1:200

            · Immunocytochemistry: 1:20-1:200

· Excitation spectrum: 490-495nm

· Emission spectrum: 520-530nm

 KLH conjugated synthetic peptide derived from human TFR

.  · Optimal working dilutions must be determined by the end user.

· protect from light.

Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

anW l= D???Opt "Times New Roman"'>         use for western blotting,elisa,immunoprecipitation and immunohistochemistry

–         Protein G affinity chromatography purification, purity :>95%

–         Isotype:  IgG

Application :  

–         WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500  KLH conjugated synthetic peptide derived from Ph Phosphoserine/threonine

–        not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user..

Storage:  Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The  lyophilized antibody is stable at room temperature for at least one month and for greater  than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent  of antibody, the antibody is stable for at least six weeks at 2-4 ℃

Important Note: This product as supplied is intended for research use only, not for use in  human, therapeutic or diagnostic applications.