亚洲国产精品二区久久,日本美女后入式午夜视频在线观看,国产污视频在线观看,欧美日韩国产精品中文字幕在线观看

上海瑞齊生物科技有限公司

Porcine SIgA

時(shí)間:2012-7-4閱讀:475
分享:

 

RD
     Porcine SIgA

FOR RESEARCH USE ONLY
Assay range1μg/ ml -40μg/ ml                    96determinations
Purpose
This kit allows for the determination ofSIgAconcentrations in Porcine serum, cellculture supernates and other biological fluids
 
Principle of the assay
The kit assay Porcine SIgAlevel in the sample,use Purified Porcine SIgAantibody to coat microtiter plate wells, make solid-phase antibody, then addPorcine SIgA to wells,Combined antibody which With HRP labeled goat anti-Porcine become antibody - antigen - enzyme-antibody complex, after washing Compley,Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed,reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of  Porcine SIgAin the samples is then determined by comparing the O.D. of the samples to the standard curve.
Materials provided with the kit

1
wash solution
20ml×1bottle
7
Stopp Solution
6ml×1 bottle
2
HRP-Conjugate reagent
6ml×1 bottle
8
Standard80μg/ ml
0.5ml×1 bottle
3
Microelisa stripplate
12well×8strips
9
Standard diluent
1.5ml×1bottle
4
Sample diluent
6ml×1 bottle
10
Instruction
1
5
Chromogen Solution A
6ml×1 bottle
11
Closure plate membrane
2
6
Chromogen Solution B
6ml×1 bottle
12
Sealed bags
1

 Porcine SIgA
 
Specimen requirements
1.       extractas soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.
2.       Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.
Assay procedure
1.       Dilute and add sample:Dilute Original density Standard as follow table:

40μg/ ml
5 Standard
150μl Original density Standard+150μl Standard diluent
20μg/ ml
4 Standard
150μl 5 Standard+150μl Standard diluent
10μg/ ml
3 Standard
150μl 4 Standard+150μl Standard diluent
5μg/ ml
2 Standard
150μl 3 Standard +150μl Standard diluent
2.5μg/ ml
1 Standard
150μl 2 Standard +150μl Standard diluent

2.add sampleSet blank wells separay (blank comparison wells don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.
3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37.
4.Configurate liquid: 30-fold wash solution diluted 30-fold (or 20-fold) with distilled water and reserve.
5.washingUncover Closure plate membrane, discardLiquid, dry by swing, add washing buffer to every well, still for 30s then drain,repeat 5 times, dry by pat.
6.add enzymeAdd HRP-Conjugate reagent 50μl to each well, except  blank well.
7.incubateOperation with 3.
8.washingOperation with 5.
9.colorAdd Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the light preservation for 15 min at 37
10.Stop the reactionAdd Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).
11.assaytake blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.
 Porcine SIgA
Steps description

Standard, Sample diluent

 

AddStandard, Sample diluent, incubate for 30 min at 37.

 

Wash 5 time,AddHRP-Conjugate reagent, incubate for 30 min at 37.

 

Wash 5 times,Add Chromogen Solution A and B, incubate for 30 min at 37.

 

AddStopp Solution

 

Read absorbance at 450nm within 15 min

 

calculate

Calculate
Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.
Important notes
1.       The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
2.       washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.
3.       add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 min, if the number of sample is much , recommend to use Volley .
4.       if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluenteand multiplied by the dilution factor.×n×5.
5.       Closure plate membrane only limits the disposable use, to avoid cross-contamination.
6.       The substrate evade the light preservation.
7.       Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.
8.       All samples, washing buffer and each kind of reject should according to infective material process.
9.       Do not mix reagents with those from other lots.
 
Storage and validity
1Storage 2-8℃.
2validity six months.
 

 

 Porcine SIgA

會(huì)員登錄

×

請(qǐng)輸入賬號(hào)

請(qǐng)輸入密碼

=

請(qǐng)輸驗(yàn)證碼

收藏該商鋪

X
該信息已收藏!
標(biāo)簽:
保存成功

(空格分隔,最多3個(gè),單個(gè)標(biāo)簽最多10個(gè)字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時(shí)間回復(fù)您~

以上信息由企業(yè)自行提供,信息內(nèi)容的真實(shí)性、準(zhǔn)確性和合法性由相關(guān)企業(yè)負(fù)責(zé),環(huán)保在線對(duì)此不承擔(dān)任何保證責(zé)任。

溫馨提示:為規(guī)避購(gòu)買風(fēng)險(xiǎn),建議您在購(gòu)買產(chǎn)品前務(wù)必確認(rèn)供應(yīng)商資質(zhì)及產(chǎn)品質(zhì)量。

在線留言
高清国产一区二区| 欧美国产综合日韩一区二区| 东京热无码AV一区二区三区| 五月天亚洲激情综合av| 国产成人AV剧情| 日本免费一区二区在线| 中文字幕亚洲欧美精品一区二区| 午夜精品福利一区二区三区蜜桃p| 国产精品碰碰现在自| 国产高欧美性情一线在线| 日韩精品人妻一区二区免费| 操世界最美丽的逼片| 91午夜福利1000集| 欧美日韩在线成人| 操逼啊 啊 啊黄色视频| 男女边吃奶边做边爱视频| 日韩一区二区三区国色天香| 国产乱色国产精品免费播放| 日韩久久奶茶视频| 国产精品熟女一区二区三区久久夜| 日本一区二区三区四区五| 日本熟妇 bbw| 亚洲男人的天堂2021| 欧美一区二区三区刘玥| 日本男人捅女人机机| 亚洲一区二区三区精品日韩| 精品日韩欧美精品日韩| 美女被大屌操大骚逼| 看一下日本人插逼逼洞视频| 国产精品久久一区二区三区夜色| 在线无码一区二区三区不卡| 把女生操出水的视频| 国产成人精品免费视频全| 国产试看精品无码中| 中文欧美亚洲欧日韩| 国产日女人视频在线观看| 精品久久久久中文字幕人| 日本一区二区不卡在线国产| 亚洲精品伦理熟女国产| 午夜福利国产三级片| 大鸡巴抽插小骚逼视频免费|