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上海瑞齊生物科技有限公司

Rabbit Anti- RECK

時(shí)間:2012-4-16閱讀:720
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Rabbit Anti- RECK
 
Number : RS-20625R 
Quantity size : 0.1ml    (dilute with pH 7.4 0.01 M PBS or antibody diluent )  
Background: RECK (reversion-inducing-cysteine-rich protein with Kazal motifs) is a membrane anchored glycoprotein that binds to and inhibits the proteolytic activity of matrix metalloproteinase-9 (MMP-9). The enzymatic activity of MMP-9 facilitates tumor invasion by proteolytically digesting the extracellular matrix, thereby enabling tumor growth, expansion and metastasis. RECK inhibits the secretion and activation of MMP-9 into the extracellular matrix, which results in the inhibition of tumor growth. RECK contains multiple EGF-like repeats and serine-protease inhibitor-like domains. The expression of RECK is suppressed  in several tumors and oncogenically transformed cells, suggesting that the loss of RECK activity correlates with transformed phenotypes. Transcriptional activation of RECK is potentially negatively regulated by the Sp1 family of transcription factors, as it contains two Sp1 binding motifs in the promoter region, and, in cells transformed with the ras oncogene, the Sp1 promoter region is essential for repressing RECK gene expression.
 Rabbit Anti- RECK
specificity :        
– Anti-RECK is a rabbit monoclonal antibody unconjugated                                  
– specific for RECK of human, mouse and rat                
– use for western blotting,elisa,immunoprecipitation and immunohistochemistry
– Protein G affinity chromatography purification, purity :>95%
– Isotype:    IgG
– mol wt:    110kDa  
 
Application :      
– Western blotting 1:100-500                                  
– Immunohistochemistry 1:100-500                                    
– ELISA 1:500-1000                                       
– Optimal working dilutions must    be determined by the end user.
 
Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃
 
Important Note: This product as supplied is intended for research use only, not for
use in human, therapeutic or diagnostic applications.
Rabbit Anti- RECK

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