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上海瑞齊生物科技有限公司

Anti- PAR-4, FITC conjugated抗體說明書

時(shí)間:2012-3-6閱讀:796
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 Anti- PAR-4, FITC conjugated抗體說明書


Quantity size : 0.2ml (2mg/ml,Lyophilized. Buffer = 0.01M PBS, pH 7.4 with 1% BSA and 0.1% Sodium azide. Reconstitute with 0.2ml sterile distilled water. )
 
Background: PAR4 is a member of a unique family of GPCRs,the proteinase-activated receptors(PARs),that are activated by proteolytic cleavage of the N-terminal domain of the receptor to reveal a tethered ligand.The PAR family consists of 4 receptors;PAR1 and APR3 are activated by thrombin,and PAR2 and PAR4 are activated by several serine proteases.PAR4 is expressed along with PAR1 on human plaets,and each plays an important role in thrombin-induced plaet aggregation.However,plaets from mice express PAR4 but not PAR1,and plaets from mice lacking PAR4 fail to aggregate in response to thrombin.Mice lacking PAR4 are protected from mesenteric arteriole thrombosis,indicating that PAR4 is a potential target for treatment of thrombosis in humans.

 Anti- PAR-4, FITC conjugated抗體說明書

Specificity :
· anti- PAR-4 is a rabbit polycional antibody, FITC conjugated
           · specific for PAR-4 of human, mouse and rat  
           · epitope mapping near the amino terminus of PAR-4 of human, mouse and rat origin  
· affinity chromatography purification, purity :>95%
· Isotype: IgG 
· mol wt:44kDa

Application : · FCM: 1:20-100
            · Immunohistochemistry : 1:20-1:200
            · Immunocytochemistry: 1:20-1:200
· Excitation spectrum: 490-495nm
· Emission spectrum: 520-530nm
· Optimal working dilutions must be determined by the end user.
· protect from light.

Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 Anti- PAR-4, FITC conjugated抗體說明書

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