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上海瑞齊生物科技有限公司

Anti-Ubiquitin/UBC/UBB 抗體說明書

時間:2012-2-7閱讀:442
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 Anti-Ubiquitin/UBC/UBB 抗體說明書

 Quantity size :   0.1ml (dilute with pH 7.4    0.01 M PBS or antibody diluent )
 
Background: Ubiquitin is a highly conserved protein, which has an ATP dependent role in the targeting of proteins for proteolytic degradation. To perform this function, the protein to be degraded is first covalently attached to the C terminus of ubiquitin, and the ubiquitinated complex is then recognized by a complex of degradative enzymes. Interestingly, ubiquitin also becomes covalently bonded to many types of pathological inclusions, which appear to be resistant to normal degradation. Therefore, ubiquitin antibodies are very useful for studies of these inclusions. For example, the neurofibrillary tangles and paired helical filaments diagnostic of Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease are all heavily ubiquitinated and can be readily visualized with ubiquitin antibodies.
Protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Attachment to proteins as a Lys-48-linked polymer usually leads to their degradation by proteasome. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous functions, including maintenance of chromatin structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair.
 

 Anti-Ubiquitin/UBC/UBB 抗體說明書

pecificity :   
        Anti- Ubiquitin is a rabbit monoclonal antibody unconjugated                
         specific for Ubiquitin of human, mouse, rat           
         use for western blotting,elisa, and Immunofluorescence
         Protein G affinity chromatography purification, purity :>95%
         Isotype:  IgG
         mol wt:  12.5 kDa
 
Application :  
        Western blotting 1:100-500                
         Immunofluorescence:100-500                 
         ELISA 1:500-1000                  
         Optimal working dilutions must be determined by the end user.
 
Storage: Store at -20 for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4
 
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 Anti-Ubiquitin/UBC/UBB 抗體說明書

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