Rabbit Anti- BACE1/ASP2
Catalog Number:Rs-0164R
Quantity size:0.1ml （dilute with pH 7.4 0.01 M PBS or diluent of antibody）
Background: Accumulation of the amyloid-b（Ab）plaque in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. Ab peptide is generated by proteolytic cleavage of the b-amyloid protein precursor （APP） at b-and g-sites by two proteases. APP is first cleaved by b-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment （C99）. The C99 fragment serves as substrate for g-secretase to generate the 4kD amyloid-b peptide, which is deposited in the brains of all suffers of Alzheimer’s disease. The long-sought b-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme （BACE） and aspartyl protease 2 （Asp2）. BACE/Asp2 is a novel transmembrane aspartic protease and colocalizes with APP. This antibody can be used for detection of BACE by Western blot at 0.5 to 1 ug/ml. Human brain tissue lysate can be used as positive control and an approximay 70kD band should be detected. It is human and mouse reactive.
Rabbit Anti- BACE1/ASP2
Specificity : ·anti-BACE1 is a rabbit monoclonal antibody unconjugated
·specific for BACE1 of human, mouse, rat
·use for western blot, elisa, immunoprecipitation
and immunohistochemistry
·affinity chromatography purification, purity :>95%
·isotype: IgG
·mol wt: 91kDa
Application :· Immunohistochemistry: 1:100-500
· ELISA: 1:500-1000
· Western blotting 1:100-500
· Optimal working dilutions must be determined by the end user.
Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ºC.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Rabbit Anti- BACE1/ASP2
