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上海瑞齊生物科技有限公司

Rabbit Anti- Estrogen receptor-beta(ER-beta)說明書

時間:2010-12-20閱讀:951
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Rabbit Anti- Estrogen receptor-beta(ER-beta)

Catalog Number :   RS-0116R
 
Quantity size :   0.1ml (dilute with pH 7.4 0.01 M PBS or antibody diluent )
 
Background:    Estrogen and progesterone receptor   are members of a family of transcription factors that are regulated by the binding of their cognate ligands. The interaction of hormone-bound estrogen receptors with estrogen responsive elements(EREs) alters transcription of ERE-containing genes. The carboxy terminal region of the estrgen receptor contains the ligand binding domain, the amino terminus serves as the transactivation domain, and the DNA binding domain is centrally located. Two forms of estrogen receptor have been identified, ERαand ERβ. ERαand ERβ have been shown to be differentially activated by various ligands. The biological
response to progesterone is mediated by two distinct forms of the human progesterone receptor (hPR-A and hPR-B), which arise from alternative splicing. In most cells, hPR-B functions as a transcriptional activator of progesterone-responsive gene, whereas hPR-A function as a transcriptional inhibitor of all steroid hormone receptors.   
           
Specificity :    · anti- ER β is a rabbit polyclonal antibody unconjugated
                ·specific for ER β of hu, mo, rat, pig, cow
                ·epitope mapping near the N-terminal of ER β of human, mo, rat, 
                ·use for western blotting, elisa, immunoprecipitation  
                   and immunohistochemistry
· Protein G affinity chromatography purification, purity :>95%
· Isotype: IgG
· mol wt: 53kDa
 
Application:    ·Western blotting 1:100-500
                 · Immunohistochemistry 1:100-500
                 · ELISA 1:500-1000
 · Optimal working dilutions must be determined by the end user.
 
Storage:     Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.
 
Important Note: This product as supplied is intended for research use only, not for use in
human, therapeutic or diagnostic applications.
 
 
 

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