Anti-GM130抗體說(shuō)明書(shū)

Catalog Number : Rs-8155R

Quantity size :   0.1ml  （dilute with pH 7.4    0.01 M PBS or antibody diluent ）

Background: GM130抗體Anti-GM130抗體說(shuō)明書(shū)

The docking of transport vesicles to their target membrane is mediated by p115. GM130, a cis-Golgi matrix protein, interacts specifically with p115 and provides a membrane docking site. Both GM130 and p115 are involved in vesicle tethering to Golgi membranes. The amino-terminus of GM130 binds to p115, whereas the carboxy-terminus binds to Golgi membranes. Both Giantin and GM130 compete for binding to p115. Thus, p115-Giantin and p115-GM130 interactions might mediate independent membrane tethering events. Transport from the ER to the cis/medial Golgi compartments requires the action of p115, GM130 and Giantin via a sequential rather than a coordinate mechanism. Mitotic phosphorylation of GM130 at Serine 25 is mediated by Cdc2, prevents binding to p115 and is directly involved in mitotic Golgi fragmentation. GM130 is phosphorylated in prophase as the Golgi complex starts to break down, and remains phosphorylated in metaphase and anaphase. In ophase, GM130 is dephosphorylated by PP2A as the Golgi fragments start to reassemble.

Subunit : Part of a larger oligomeric complex. Interacts with p115. Interacts with RAB1B that has been activated by GTP-binding. Interacts with GORASP1/GRASP65 and ZFPL1.Subcellular Location : Golgi apparatus, Golgi stack membrane; Peripheral membrane protein.Similarity : Belongs to the GOLGA2 family.

pecificity :   

-  Anti-GM130 is a  Rabbit  polyclonal antibody unconjugated               

specific for GM130 of   Human, Mouse, Rat, Pig, Cow 

• use for western blotting,elisa,immunoprecipitation and immunohistochemistry
• Protein G affinity chromatography purification, purity :>95%
• Isotype:  IgG
• mol wt:110kDa

Application :  

• WB=1:100-500 ELISA=1:500-1000 IP=1:20-100  IHC-P=1:100-500 IHC-F=1:100-500  IF=1:100-500
  not yet tested in other applications.
• optimal dilutions/concentrations should be determined by the end user..

Storage:  Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The  lyophilized antibody is stable at room temperature for at least one month and for greater  than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent  of antibody, the antibody is stable for at least six weeks at 2-4 ℃

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.GM130抗體Anti-GM130抗體說(shuō)明書(shū)