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改良磷酸鹽緩沖液 PSB

Phosphate Saline Buffer,Modified

250g

用于小腸結(jié)腸炎耶爾森氏菌增菌培養(yǎng) （GB標(biāo)準(zhǔn)）

用途：用于小腸結(jié)腸炎耶爾森氏菌增菌培養(yǎng)

改良磷酸鹽緩沖液 PSB 配方：（g/L）

磷酸氫二鈉
磷酸二氫鈉
山梨醇
氯化鈉
3號(hào)膽鹽
pH值7.6 ± 0.2

8.23
1.2
10.0
5.0
1.5
25℃

原理：

山梨醇提供碳氮源，磷酸氫二鈉和磷酸二氫鈉為緩沖液，膽鹽抑制革蘭氏陽性菌，氯化鈉提供細(xì)胞所需要的滲透壓。

用法：

稱取本品 25.93g ,加熱攪拌溶解于 1000ml 蒸餾水中,分裝 ,每瓶 225ml,121℃ 高壓滅菌 15 分鐘備用。

質(zhì)量控制：

在25℃培養(yǎng)18-24小時(shí)

質(zhì)控菌株	ATCC	生長情況
小腸結(jié)腸炎耶爾森氏菌		（10-7）+++
金葡萄球菌	25923	-
大腸桿菌	25922	（10-7）+++
沙門氏菌	14028	（10-7）+++

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