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重組大鼠白介素-3/IL 3 Rat

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重組大鼠白介素-3/IL 3 Rat產(chǎn)品編號(hào)：CYT-383
產(chǎn)品規(guī)格：5μg/20μg/1mg

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重組大鼠白介素-3/IL 3 Rat

產(chǎn)品編號(hào)：CYT-383
產(chǎn)品規(guī)格：5μg/20μg/1mg
 

 

Catalogue Number CYT-383
Synonyms MCGF （Mast cell growth factor）, Multi-CSF, HCGF, P-cell stimulation factor, IL-3, MGC79398, MGC79399.
Introduction Interleukin-3 is a pleiotropic cytokine produced primarily by activated T cells.
IL-3 is thought to function via specific cell surface receptors to stimulate the proliferation, differentiation and survival of haematopoietic cell lines. IL-3 has also been shown to affect the functional activity of a variety of other cell types including mast cells, eosinophils, megakaryocytes and basophils.
Description Interleukin-3 Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 140 amino acids and having a molecular mass of 16 kDa.
The IL-3 is purified by proprietary chromatographic techniques.
Source Escherichia Coli.
Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder.
Formulation Lyophilized from a 1mg/ml solution without any additives.
Solubility It is recommended to reconstitute the lyophilized Interleukin 3 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized Interleukin-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL3 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles.
Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE.
Amino acid sequence The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Asp-Arg-Gly.
Biological Activity The ED50= 3-10 ng/ml corresponding to a specific activity of 100,000-333,334IU/mg determined by the dose dependent proliferation of murine NFS-60 cells.
Protein content Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 0.171 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）.

2. Analysis by RP-HPLC, using a standard solution of IL-3 as a Reference Standard.
Usage ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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