人6-羥多巴胺(6-OHDA)ELISA試劑盒英文名稱Human 6-hydroxydopamine,6-OHDA ELISA試劑盒齊一生物科技有限公司主要經(jīng)營進口原裝Elisa試劑盒,種類齊全,價格實惠,貨期短.購買我公司產(chǎn)品新老客戶,可享全程技術指導:(包括售前標本收集、后期實驗、實驗結束后數(shù)據(jù)分析等技術問題,我公司會有專業(yè)的技術即時給您去電溝通、解決.)
人6-羥多巴胺(6-OHDA)ELISA試劑盒有效期:6個月
預期應用:ELISA法定量測定血清、血漿、細胞培養(yǎng)上清或其它相關生物液體中的含量.
說明
1.試劑盒保存:-20℃(較長時間不用時);2-8℃(頻繁使用時).
2.濃洗滌液低溫保存會有鹽析出,稀釋時可在水浴中加溫助溶.
3.中、英文說明書可能會有不*之處,請以英文說明書為準.
4.剛開啟的酶聯(lián)板孔中可能會含有少許水樣物質,此為正?,F(xiàn)象,不會對實驗結果造成任何影響.
實驗原理
用純化的抗體包被微孔板,制成固相載體,往包被抗該指標抗體的微孔中依次加入標本或標準品、*化的抗該指標抗體、HRP標記的親和素,經(jīng)過*洗滌后用底物TMB顯色.TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成zui終的黃色.顏色的深淺和樣品中的該指標呈正相關.用酶標儀在450nm波長下測定吸光度(OD值),計算樣品濃度.
試劑盒組成及試劑配制
1. 酶聯(lián)板(Assay plate ):一塊(96孔).
2. 標準品(Standard):2瓶(凍干品).
3. 樣品稀釋液(Sample Diluent):1×20ml/瓶.
4. *標記抗體稀釋液(Biotin-antibody Diluent):1×10ml/瓶.
5. 辣根過氧化物酶標記親和素稀釋液 (HRP-avidin Diluent):1×10ml/瓶.
6. *標記抗體(Biotin-antibody):1×120μl/瓶(1:100)
7. 辣根過氧化物酶標記親和素(HRP-avidin):1×120μl/瓶(1:100)
8. 底物溶液(TMB Substrate):1×10ml/瓶.
9. 濃洗滌液(Wash Buffer):1×20ml/瓶,使用時每瓶用蒸餾水稀釋25倍.
10. 終止液(Stop Solution):1×10ml/瓶(2N H2SO4).
10、標本的采集及保存
1. 血清:全血標本請于室溫放置2小時或4℃隔夜后于1000 x g離心20分鐘,取上清即可檢測,或將標本放于-20℃或-80℃保存,但應避免反復凍融.
2. 血漿:可用EDTA或肝素作為抗凝劑,標本采集后30分鐘內(nèi)于2 - 8° C 1000 x g離心15分鐘,或將標本放于-20℃或-80℃保存,但應避免反復凍融.
3. 細胞培養(yǎng)物上清或其它生物標本:1000 x g離心20分鐘,取上清即可檢測,或將標本放于-20℃或-80℃保存,但應避免反復凍融.
注:標本溶血會影響zui后檢測結果,因此溶血標本不宜進行此項檢測.
31630 ImmunoPure (R) Fluorescein Conjugated Goat Anti-Mouse IgG 1 MG
31634 ImmunoPure (R) Fluorescein Conjugated Goat Anti-Mouse IgG 0.5 MG
31635 ImmunoPure (R) Fluorescein Conjugated Goat Anti-Rabbit 2 MG
31636 ImmunoPure (R) Fluorescein Conjugated F(ab')2 Fragment 1 MG
31650 Pierce(R) Rhodamine Conjugated Rabbit Anti-Goat IgG (H+L), 1.5 MG
31656 Pierce(R) Rhodamine Conjugated Goat Anti-Human IgG (H+L), 2 MG
31660 Pierce(R) Rhodamine Conjugated Goat Anti-Mouse IgG (H+L), 2 2 MG
31661 Pierce(R) Rhodamine Conjugated Goat Anti-Mouse IgG (H+L) 1.5 MG
31663 Pierce(R) Rhodamine Conjugated Goat Anti-Mouse IgG (Fc) 2.0 MG
31665 Pierce(R) Rhodamine Conjugated Rabbit Anti-Mouse IgG (H+L) 1.5 MG
31670 Pierce(R) Rhodamine Conjugated Goat Anti-Rabbit IgG (H+L), 2 MG
31680 Pierce(R) Rhodamine Conjugated Goat Anti-Rat IgG(H+L), 2 MG 2 MG
31683 ImmunoPure (R) Rhodamine Conjugated Goat Anti-Human IgG 1.5 MG
31684 ImmunoPure (R) Rhodamine Conjugated Goat Anti-Human IgG 2 MG
31685 ImmunoPure (R) Rhodamine Conjugated Donkey Anti-Rabbit 0.5 MG.
31686 ImmunoPure (R) Rhodamine Conjugated Goat Anti-Rabbit 1.5 MG.
31687 ImmunoPure (R) Rhodamine Conjugated Mouse Monoclonal 0.5 MG.
31700 Peptide Retention Standard 1 vial
31720 Pierce(R) Biotin Conjugated Rabbit Anti-Chicken IgY (H+L), 1.5 ML
31730 Pierce(R) Biotin Conjugated Mouse Anti-Goat IgG (H+L)(min X 1 ML
31732 Pierce(R) Biotin Conjugated Rabbit Anti-Goat IgG (H+L), 1.5 1.5 MG
31750 Pierce(R) Biotin Conjugated Goat Anti-Hamster IgG (H+L), 1. 1.5 MG
31753 Pierce(R) Biotin Conjugated Rabbit Anti-Goat IgG {F(ab')2} 1.5 ML
31760 Pierce(R) Biotin Conjugated Goat Anti-Horse IgG (H+L), 1.5 1.5 MG
31770 Pierce(R) Biotin Conjugated Goat Anti-Human IgG(H+L), 1.5 M 1.5 MG
31774 Pierce(R) Biotin Conjugated Goat Anti-Human IgG (H+L)(min X 1.5 ML
31778 Pierce(R) Biotin Conjugated Goat Anti-Human IgMu, 0.5 MG 0.5 MG
31780 Pierce(R) Biotin Conjugated Goat Anti-Human IgG (KAPPA CHAIN 0.5 MG
31782 Pierce(R) Biotin Conjugated Goat Anti-Human IgA+IgG+IgM (H+L 2 ML
31784 Pierce(R) Biotin Conjugated Mouse Anti-Human IgG (H+L)(min 1 ML
31786 Pierce(R) Biotin Conjugated Rabbit Anti-Human IgG (H+L), 1.5 1.5 ML
31789 Pierce(R) Biotin Conjugated Rabbit Anti-Human IgG (Fc) 1.5 ML
31800 Pierce(R) Biotin Conjugated Goat Anti-Mouse IgG (H+L), 2 ML
31802 Pierce(R) Biotin Conjugated Goat Anti-Mouse IgG (H+L), 1.5 ML
31803 Pierce(R) Biotin Conjugated Goat Anti-Mouse IgG {F(ab')2} 2.0 ML
31804 Pierce(R) Biotin Conjugated Goat Anti-Mouse IgM, (mu Chain 0.5 MG
31805 Pierce(R) Biotin Conjugated Goat Anti-Mouse IgG (Fc) 2.0 ML
31806 Pierce(R) Biotin Conjugated Horse Anti-Mouse IgG(H+L), 1.5 MG
31807 Pierce(R) Biotin Conjugated Goat Anti-Mouse IgG + IgM (H+L) 2.0 ML
31813 Pierce(R) Biotin Conjugated Rabbit Anti-Mouse IgG (Fc) 1.5 ML
31820 Pierce(R) Biotin Conjugated Goat Anti-Rabbit IgG (H+L), 1.5 1.5 MG
31821 Pierce(R) Biotin Conj. Donkey Anti-Rabbit IgG(H+L)(minxBvCh 0.5 ML
31822 Pierce(R) Biotin Conjugated Goat Anti-Rabbit IgG (H+L) 1.5 ML
31823 Pierce(R) Biotin Conjugated Goat Anti-Rabbit IgG {F(ab')2} 2.0 ML
31824 Pierce(R) Biotin Conjugated Mouse Anti-Rabbit IgG (H+L)(min 1 ML
31830 IMMUNOPURE BIOTIN CONJUGATED GOAT ANTI-RAT IgG (H+L), 2 ML 2 ML
31832 Pierce(R) Biotin Conjugated Goat Anti-Rat IgMu, 2 ML 2 ML
31834 Pierce(R) Biotin Conjugated Rabbit Anti-Rat IgG (H+L), 1.5 1.5 MG
31840 Pierce(R) Biotin Conjugated Rabbit Anti-Sheep IgG (H+L), 1. 1.5 MG
31850 Biotinylated Goat Anti-Avidin 0.5 MG
31852 Pierce Goat Anti-Biotin 1.0 MG
31860 ImmunoPure (R) R-Phycoerythrin Conjugated F(ab')2 Fragment 1 ML
31861 ImmunoPure (R) R-Phycoerythrin Conjugated Goat Anti-Mouse 1 ML
31862 ImmunoPure (R) R-Phycoerythrin Conjugated Goat Anti-Mouse 0.5 ML
31863 ImmunoPure (R) R-Phycoerythrin Conjugated Goat Anti-Mouse 0.5 ML
31864 ImmunoPure (R) R-Phycoerythrin Conjugated F(ab')2 Fragment 1 ML
31871 Pierce(R) Goat Gamma Globulin, 10 mg. 10 MG
31878 IMMUNOPURE MOUSE GAMMA GLOBULIN, 10 MG 10 MG
31879 Human Gamma Globulin, 10 mg. 10 MG
31885 Pierce(R) Rat Gamma Globulin, 10 mg 10 MG
31887 Pierce(R) Rabbit Gamma Globulin, 10 mg 10 MG
31903 Mouse IgG, Whole Molecule, 5 mg. 5 MG
31933 Rat IgG, Whole Molecule 10 MG
31981 ImmunoPure (R) Allophycocyanin Conjugated Goat Anti-Mouse 0.5 ML
31982 ImmunoPure (R) Allophycocyanin Conjugated Goat Anti-Mouse 0.3 ML
31983 ImmunoPure (R) Allophycocyanin Conjugated Goat Anti-Mouse 0.3 ML
31984 ImmunoPure (R) Allophycocyanin Conjugated F(ab')2 Fragment 0.5 ML
31992 Pierce(R) Fluorescein Conjugated Goat Anti-Mouse IgMu 2 MG
32020 ABC Standard Peroxidase Staining Kit Kit
32050 ABC Ultra-Sensitive Peroxidase Staining Kit Kit
12381 EndoFree Plasmid Mega Kit (5)
12391 EndoFree Plasmid Giga Kit (5)
28104 QIAquick PCR Purification Kit (50)
28106 QIAquick PCR Purification Kit (250)
28704 QIAquick Gel Extraction Kit (50)
28706 QIAquick Gel Extraction Kit (250)
貨號 品名
51104 QIAamp DNA Blood Mini Kit (50)
51106 QIAamp DNA Blood Mini Kit (250)
人6-羥多巴胺(6-OHDA)ELISA試劑盒28004 MinElute PCR Purification Kit (50)
28006 MinElute PCR Purification Kit (250)
28304 QIAquick Nucleotide Removal Kit (50)
28306 QIAquick Nucleotide Removal Kit (250)
56404 QIAamp DNA FFPE Tissue Kit (50)
217004 miRNeasy Mini Kit (50)
210212 QIAGEN OneStep RT-PCR Kit (100)
59104 EpiTect Bisulfite Kit (48)
貨號 品名
27104 QIAprep Spin Miniprep Kit (50)
69104 DNeasy Plant Mini Kit (50)
69106 DNeasy Plant Mini Kit (250)
51304 QIAamp DNA Mini Kit (50)
27106 QIAprep Spin Miniprep Kit (250)
51183 QIAamp DNA Blood Midi Kit (20)
51185 QIAamp DNA Blood Midi Kit (100)
51306 QIAamp DNA Mini Kit (250)
52904 QIAamp Viral RNA Mini Kit (50)
52906 QIAamp Viral RNA Mini Kit (250)
69504 DNeasy Blood & Tissue Kit (50)
69506 DNeasy Blood & Tissue Kit (250)
30210 Ni-NTA Agarose (25 ml)
70022 Oligotex mRNA Mini Kit (12)
74104 RNeasy Mini Kit (50)
74106 RNeasy Mini Kit (250)
74903 RNeasy Plant Mini Kit (20)
74904 RNeasy Plant Mini Kit (50)
76104 RNAlater (50ml)
76106 RNAlater (250ml)
79254 RNase-Free DNase Set (50)
74134 RNeasy Plus Mini Kit (50)
51504 QIAamp DNA Stool Mini Kit (50)
51604 QIAamp Fast DNA Stool Mini Kit (50)