細(xì)胞名稱

RW.4小鼠胚胎干細(xì)胞RW.4（干細(xì)胞庫保藏）

描述

小鼠*性胚胎干細(xì)胞，每支細(xì)胞量1×10⁶

動物種別

小鼠，129X1/SvJ品系

性別

雄

組織來源

小鼠胚胎內(nèi)細(xì)胞團(tuán)

形態(tài)

球形克隆

培養(yǎng)基和添加劑

mES*培養(yǎng)液配方（600 ml）： DMEM (Invitrogen 12430) 497 ml ES級FBS (biochrom S4115) 90 ml Glutamax (Invitrogen 35050) 6 ml NEAA (Invitrogen 11140) 6 ml LIF (Millipore ESG1107) 60 μl（終濃度為1000U/ml） β-Mer (Invitrogen 21985) 1.5 ml 氣相：空氣，95%；二氧化碳，5%。溫度：37攝氏度。

供應(yīng)限制

REFERENCE

Hug BA, et al. Analysis of mice containing a targeted deletion of beta-globin locus control region 5' hypersensitive site 3. Mol. Cell. Biol. 16: 2906-2912, 1996. PubMed: 8649401 Matise MP, et al. Production of targeted Embryonic Stem Cell Clones. In AL Joyner (Ed.), Gene Targeting: A Practical Approach. Oxford University Press, Oxford, 101-132, 1999. Fabricius D, Bonde S, Zavazava N. Induction of stable mixed chimerism by embryonic stem cells requires functional Fas/FasL engagement. Transplantation 79(9): 1040-1044, 2005. PubMed:15880040 Conte D, et al. Inhibitor of apoptosis protein cIAP2 is essential for lipopolysaccharide-induced macrophage survival. Mol. Cell Biol. 26(2): 699-708, 2006. PubMed: 16382159 Westervelt P, et al. High-penetrance mouse model of acute promyelocytic leukemia with very low levels of PML-RARalpha expression. Blood 102(5): 1857-1865, 2003. PubMed: 12750176 Schumacher A, et al. Staurosporine is a potent activator of neuronal, glial, and "CNS stem cell-like" neurosphere differentiation in murine embryonic stem cells. Mol. Cell Neurosci. 23: 669-680, 2003. PubMed: 12932446 Lee MH, et al. Simvastatin suppresses self-renewal of mouse embryonic stem cells by inhibiting RhoA geranylgeranylation. Stem Cells 25: 1654-1663, 2007. PubMed: 17464088