細(xì)胞名稱

C2C12小鼠胚胎肌母細(xì)胞（未分化）（干細(xì)胞庫保藏）

描述

小鼠胚胎肌母細(xì)胞

動物種別

小鼠 品系C3H

組織來源

肌肉

形態(tài)

肌母細(xì)胞狀

培養(yǎng)基和添加劑

DMEM Medium (Invitrogen, 12430-054) 90 ml 　　 FBS (Biochrom, S0115) 10 ml 氣相：空氣，95%；二氧化碳，5%。溫度：37攝氏度。

供應(yīng)限制

REFERENCE

Yaffe D, Saxel O. Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle. Nature 270: 725-727, 1977. PubMed: 563524 Blau HM, et al. Plasticity of the differentiated state. Science 230: 758-766, 1985. PubMed: 2414846 Katagiri T, et al. Bone morphogenetic protein-2 converts the differentiation pathway of C2C12 myoblasts into the osteoblast lineage [published erratum appears in J Cell Biol 1995 Feb;128(4):following 713]. J. Cell Biol. 127: 1755-1766, 1994. PubMed: 7798324 Chow YH, et al. Improvement of hepatitis B virus DNA vaccines by plasmids coexpressing hepatitis B surface antigen and interleukin-2. J. Virol. 71: 169-178, 1997. PubMed: 8985336 Kessler PD, et al. Gene delivery to skeletal muscle results in sustanined expression and systemic delivery of a therapeutic protein. Proc. Natl. Acad. Sci. USA 93: 14082-14087, 1996. PubMed: 8943064 Hsu DK, et al. Identification of a murine TEF-1-related gene expressed after mitogenic stimulation of quiescent fibroblasts and during myogenic differentiation. J. Biol. Chem. 271: 13786-13795, 1996. PubMed: 8662936 Qing Y, et al. Inhibitory effects of iron on bone morphogenetic protein 2-induced osteoblastogenesis. J. Bone Miner. Res. 26(6): 1188-1196, 2011. PubMed: 21308772