細胞名稱 | NIH/3T3小鼠胚胎細胞 |
描述 | 與原始的隨機交配3T3和近交系BALB/c 3T3建株方法一樣，NIH/3T3，是從NIH Swiss小鼠胚胎培養(yǎng)物中建立的高度接觸抑制的連續(xù)細胞株。 為了培育在形態(tài)學特征上更適合于進行轉化分析的亞株，建立的NIH/3T3細胞株又進行了五輪以上亞克隆。 這株細胞對DNA轉化及轉染研究十分有用。 檢測表明肢骨發(fā)育畸形病毒(鼠痘)陰性。 |
動物種別 | 小鼠 |
性別 | *** |
組織來源 | 胚胎 |
形態(tài) | 成纖維細胞 |
培養(yǎng)基和添加劑 | DMEM培養(yǎng)基(GIBCO,貨號12800017，添加NaHCO3 1.5g/L)，90%；優(yōu)質(zhì)胎牛血清，10%。 氣相：空氣，95%；二氧化碳，5%。 溫度：37攝氏度。 |
供應限制 | A。僅供研究之用。 |
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