細(xì)胞名稱

C2C12小鼠成肌細(xì)胞

描述

這是D. Yaffe 和 O. Saxel建立的小鼠成肌細(xì)胞株的亞克隆(由H. Blau等人構(gòu)建)。C2C12細(xì)胞株分化很快，形成可伸縮的肌管并生成特征性的肌蛋白。 用骨形成蛋白2(BMP-2)處理，導(dǎo)致分化途徑從成肌細(xì)胞轉(zhuǎn)換成成骨細(xì)胞。 檢測(cè)表明肢骨發(fā)育畸形病毒(鼠痘)陰性。

動(dòng)物種別

小鼠

性別

***

組織來源

組織： 肌肉 品系： C3H 細(xì)胞類型： 成肌細(xì)胞。

形態(tài)

成肌細(xì)胞

培養(yǎng)基和添加劑

DMEM培養(yǎng)基(GIBCO,貨號(hào)12800017，添加NaHCO3 1.5g/L)，90%；優(yōu)質(zhì)胎牛血清，10%。 氣相：空氣，95%；二氧化碳，5%。 溫度：37攝氏度。

供應(yīng)限制

A。僅供研究之用。

REFERENCE

Yaffe D, Saxel O. Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle. Nature 270: 725-727, 1977. PubMed: 563524 Blau HM, et al. Plasticity of the differentiated state. Science 230: 758-766, 1985. PubMed: 2414846 Katagiri T, et al. Bone morphogenetic protein-2 converts the differentiation pathway of C2C12 myoblasts into the osteoblast lineage [published erratum appears in J Cell Biol 1995 Feb;128(4):following 713]. J. Cell Biol. 127: 1755-1766, 1994. PubMed: 7798324 Chow YH, et al. Improvement of hepatitis B virus DNA vaccines by plasmids coexpressing hepatitis B surface antigen and interleukin-2. J. Virol. 71: 169-178, 1997. PubMed: 8985336 Kessler PD, et al. Gene delivery to skeletal muscle results in sustanined expression and systemic delivery of a therapeutic protein. Proc. Natl. Acad. Sci. USA 93: 14082-14087, 1996. PubMed: 8943064 Hsu DK, et al. Identification of a murine TEF-1-related gene expressed after mitogenic stimulation of quiescent fibroblasts and during myogenic differentiation. J. Biol. Chem. 271: 13786-13795, 1996. PubMed: 8662936