細(xì)胞名稱

Beta-TC-6小鼠胰島素瘤胰島β細(xì)胞

描述

這株細(xì)胞來源于轉(zhuǎn)基因小鼠中生長的一個胰腫瘤(胰島素瘤)。 這種小鼠攜帶了大鼠胰島素II基因啟動子調(diào)控的SV40早期基因的假基因結(jié)構(gòu)。 細(xì)胞包含豐富的胰島素和小量的胰高血糖素及*。 響應(yīng)葡萄糖而分泌胰島素[PubMed:7533732]。

動物種別

小鼠

性別

***

組織來源

胰；胰島素瘤；β細(xì)胞

形態(tài)

上皮細(xì)胞樣

培養(yǎng)基和添加劑

DMEM培養(yǎng)基(GIBCO,貨號12800017，添加NaHCO3 1.5g/L)，85%；優(yōu)質(zhì)熱滅活胎牛血清，15%。 氣相：空氣，95%；二氧化碳，5%。 溫度：37攝氏度。

供應(yīng)限制

A。僅供研究之用。

REFERENCE

Laurance ME , et al. Glucose responsive insulin secreting beta-cell lines and method for producing same. US Patent 5,773,255 dated Jun 30 1998 Poitout V , et al. Morphological and functional characterization of beta TC-6 cells--an insulin-secreting cell line derived from transgenic mice. Diabetes 44: 306-313, 1995. PubMed: 7533732 Poitout V , et al. Insulin-secreting cell lines: classification, characteristics and potential applications. Diabetes Metab. 22: 7-14, 1996. PubMed: 8697299 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Centers for Disease Control (1993), Biosafety in Microbiological and Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S. Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C.