細(xì)胞名稱

RAG小鼠腎腺癌細(xì)胞

描述

這是一株腎臟-2α細(xì)胞株的非回復(fù)8-氮雜*抗性(HPRT-, HGPRT-)克隆。 測(cè)試發(fā)現(xiàn)缺肢畸形病毒(鼠痘)陰性。

動(dòng)物種別

小鼠

性別

***

組織來源

腎腺癌；腎

形態(tài)

變形蟲樣

培養(yǎng)基和添加劑

MEM培養(yǎng)基(GIBCO,貨號(hào)41500034，添加NaHCO3 1.5g/L，Sodium Pyruvate 0.11g/L)，90%；優(yōu)質(zhì)胎牛血清，10%。 氣相：空氣，95%；二氧化碳，5%。 溫度：37攝氏度。

供應(yīng)限制

A。僅供研究之用。

REFERENCE

Klebe RJ , et al. Mapping of a human genetic regulator element by somatic cell genetic analysis. Proc. Natl. Acad. Sci. USA 66: 1220-1227, 1970. PubMed: 4920091 Science 145: 709, 1964. Klebe RJ , et al. Controlled production of proliferating somatic cell hybrids. J. Cell Biol. 45: 74-82, 1970. PubMed: 4318843 Felluga B , et al. Electron microscope observations on virus particles associated with a transplantable renal adenocarcinoma in BALB-cf-Cd mice. J. Natl. Cancer Inst. 43: 319-333, 1969. PubMed: 5797837 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Centers for Disease Control (1993), Biosafety in Microbiological and Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S. Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C.