細(xì)胞名稱

Neuro-2a [N2a; Neuro-2a]小鼠腦神經(jīng)瘤細(xì)胞

描述

克隆Neuro-2A是由R.J.Klebe和F.H.Ruddle經(jīng)A株白鼠的自生腫瘤建立。 該細(xì)胞產(chǎn)生大量微管蛋白，此蛋白在神經(jīng)細(xì)胞中起應(yīng)答軸漿流動(dòng)的收縮系統(tǒng)作用。

動(dòng)物種別

小鼠

性別

不明

組織來源

腦神經(jīng)母細(xì)胞瘤，神經(jīng)母細(xì)胞

形態(tài)

神經(jīng)和阿米巴樣干細(xì)胞

培養(yǎng)基和添加劑

MEM培養(yǎng)基(GIBCO,貨號(hào)41500034，添加NaHCO3 1.5g/L，Sodium Pyruvate 0.11g/L)，90%；優(yōu)質(zhì)胎牛血清，10%。 氣相：空氣，95%；二氧化碳，5%。 溫度：37攝氏度。

供應(yīng)限制

REFERENCE

Olmsted JB , et al. Isolation of microtubule protein from cultured mouse neuroblastoma cells. Proc. Natl. Acad. Sci. USA 65: 129-136, 1970. PubMed: 5263744 Klebe RJ , Ruddle FH . Neuroblastoma: Cell culture analysis of a differentiating stem cell system. J. Cell Biol. 43: 69A, 1969. Naslavsky N , et al. Characterization of detergent-insoluble complexes containing the cellular prion protein and its scrapie isoform. J. Biol. Chem. 272: 6324-6331, 1997. PubMed: 9045652 Kaneko K , et al. Evidence for protein X binding to a discontinuous epitope on the cellular prion protein during scrapie prion propagation. Proc. Natl. Acad. Sci. USA 94: 10069-10074, 1997. PubMed: 9294164 Maestrini E , et al. A family of transmembrane proteins with homology to the MET-hepatocyte growth factor receptor. Proc. Natl. Acad. Sci. USA 93: 674-678, 1996. PubMed: 8570614