細(xì)胞名稱(chēng)

HFF-1人皮膚成纖維細(xì)胞（干細(xì)胞庫(kù)保藏）

描述

白人（新生兒）成纖維細(xì)胞，P16代飼養(yǎng)層細(xì)胞，每支細(xì)胞量1×10⁶，供HN4、HN1使用。

動(dòng)物種別

人

性別

新生男孩

組織來(lái)源

取白人新生男孩的前皮制備獲得

形態(tài)

成纖維細(xì)胞

培養(yǎng)基和添加劑

DMEM ( Invitrogen, 12430) 84 ml FBS ( Biochrom, S4115) 15 ml Sodium Pyruvate ( Invitrogen, 11360-070) 氣相：空氣，95%；二氧化碳，5%。溫度：37攝氏度。

供應(yīng)限制

REFERENCE

Amit M, et al. Human feeder layers for human embryonic stem cells. Biol. Reprod. 68: 2150-2156, 2003. PubMed: 12606388 Hovatta O, et al. A culture system using human foreskin fibroblasts as feeder cells allows production of human embryonic stem cells. Hum. Reprod. 18: 1404-1408, 2003. PubMed:12832363 Andrews P, et al. Human embryonic fibroblast feeder cells. International Patent Application WO 03/078611 A1 Freshney RI. Culture of Animal Cells: A Manual of Basic Technique, 5th edition. New York: Wiley Liss; 2005. For more information on enzymatic dissociation and subculturing of cell lines see Chapter 13.