細(xì)胞名稱

NCI-H460 [H460]人大細(xì)胞肺癌細(xì)胞

描述

NCI-H460細(xì)胞株從一位大細(xì)胞肺癌患者的胸水中建立。 細(xì)胞表達(dá)的p53 mRNA易于檢測，其水平與正常肺細(xì)胞相當(dāng)，未表現(xiàn)出DNA總體結(jié)構(gòu)異常。 角蛋白和波形蛋白纖維染色陽性，神經(jīng)絲三聯(lián)體蛋白陰性。

動物種別

人

性別

男

組織來源

肺；轉(zhuǎn)移灶：肋膜滲出癌；大細(xì)胞肺癌

形態(tài)

epithelial

培養(yǎng)基和添加劑

RPMI-1640(GIBCO,貨號31800022, 添加NaHCO3 1.5g/L, glucose 2.5g/L, Sodium Pyruvate 0.11g/L)，90%；優(yōu)質(zhì)胎牛血清，10%。 氣相：空氣，95%；二氧化碳，5%。 溫度：37攝氏度。

供應(yīng)限制

REFERENCE

Banks-Schlegel SP , et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. PubMed: 2578876 Takahashi T , et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494 Brower M , et al. Growth of cell lines and clinical specimens of human non-small cell lung cancer in a serum-free defined medium. Cancer Res. 46: 798-806, 1986. PubMed: 3940644 Geiger T , et al. Antitumor activity of a PKC-alpha antisense oligonucleotide in combination with standard chemotherapeutic agents against various human tumors transplanted into nude mice. Anticancer Drug Des. 13: 35-45, 1998. PubMed: 9474241