細(xì)胞名稱

BEAS-2B 暫不供應(yīng)人正常肺上皮細(xì)胞

描述

從一位非癌個體的正常人支氣管上皮病理切片分離出上皮細(xì)胞。 這些細(xì)胞用腺病毒12-SV40病毒雜交病毒感染并克隆。 DEAS-2B細(xì)胞保留了對血清反應(yīng)進(jìn)行鱗關(guān)分化的能力，并有用于篩選誘導(dǎo)或影響分化及致癌的化學(xué)或生物制劑。 細(xì)胞角蛋白及SV40抗原染色陽性。

動物種別

人

性別

未知

組織來源

肺;支氣管;正常;上皮;病毒轉(zhuǎn)化

形態(tài)

上皮細(xì)胞樣

培養(yǎng)基和添加劑

訂購此細(xì)胞需提供100ml培養(yǎng)基。LHC-9培養(yǎng)液添加0.5鈉克/毫升重組上皮細(xì)胞生長因子(EGF),500鈉克/毫升氫化可的*，0.035鈉克/毫升牛垂體提取物，500mM乙醇胺，500nM 磷酸乙醇胺，0.01毫克/毫升腎上腺素，0.1鈉克/毫升retinoic酸及微量元素。注意：不要過濾EGF。LHC-9基礎(chǔ)培養(yǎng)液可以從Biofluids, Inc., Rockville, MD(目錄號118)或Clonetics Corporation, Walkersville, MD 21793(BEBM目錄

供應(yīng)限制

REFERENCE

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